Design and Production of Human Monoclonal Antibodies to Human Cancers

  • Mark C. Glassy
  • Harold H. Handley
  • Ivor Royston


Central questions of the tumor immunologist pertain to the type and nature of antigens appearing in the malignant state and methods used to detect them. Monoclonal antibody (MAb) technology (Köhler and Milstein, 1975) provides investigators an opportunity to study virtually any immunogenic molecule with exact precision and has been successful in detecting human tumor antigens (Boss et al., 1983). Recently, hybridoma technology has been extended to the production of human MAbs (Olsson and Kaplan, 1980; Schlom et al., 1980; Croce et al., 1980; Glassy et al., 1981, 1983a; Sikora and Phillips, 1981; Edwards et al., 1982) and is being used to study human tumor antigens.


Tumor Antigen Human Monoclonal Antibody Benign Prostatic Hypertrophy Prostate Carcinoma Cell Fusion Frequency 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. Abrams, P. G., Knost, J. A., Clarke, G., Wilburn, S., Oldham, R. K., and Foon, K. A., 1983, Determination of the optimal human cell lines for development of human hybridomas, J. Immunol. 131: 1201.PubMedGoogle Scholar
  2. Boss, B. D., Langman, R., Trowbridge, I., and Dulbecco, R. (eds.), 1983, Monoclonal Antibodies and Cancer, Academic Press, New York.Google Scholar
  3. Carey, T. E., Takahashi, T., Resnick, L. A., Oettgen, H. F., and Old, L. J., 1976, Cell surface antigens of human malignant melanoma. I. Mixed hemadsorption assay for humoral immunity to cultured autologous melanoma cells, Proc. Natl. Acad. Sci. USA 73: 3278.PubMedCrossRefGoogle Scholar
  4. Chiorazzi, N., Wasserman, R. L., and Kunkel, H. G., 1982, Use of Epstein—Barr virus-transformed B cell lines for the generation of immunoglobulin-producing human B cell hybridomas, J. Exp. Med. 156: 930.PubMedCrossRefGoogle Scholar
  5. Cleveland, P. H., Richman, D., Oxman, M. N., Wickham, M. G., Binder, P. S., and Worthen, D. M., 1979, Immobilization of viral antigens on filter paper for a (125I) staphylococcal protein A immunoassay, J. Immunol. Meth. 29: 369.CrossRefGoogle Scholar
  6. Cote, R. J., Morrissey, D. M., Houghton, A. N., Beattie, Jr., E. J., Oettgen, H. F., and Old, L. J., 1983, Generation of human monoclonal antibodies reactive with cellular antigens, Proc. Natl. Acad. Sci. USA 80: 2026.PubMedCrossRefGoogle Scholar
  7. Croce, C., Linnenbach, A., Hall, W., Steplewski, Z., and Koprowski, H., 1980, Production of human hybridomas secreting antibody to measles virus, Nature 288: 488.PubMedCrossRefGoogle Scholar
  8. Dillman, R. 0., 1984, Monoclonal antibodies in the treatment of cancer, CRC Crit. Rev. Oncol.lHematol. 1:357.Google Scholar
  9. Edwards, P. A. W., Smith, C. M., Neville, A. M., and O’Hare, M. J., 1982, A human—human hybridoma system based on a fast-growing mutant of the ARH-77 plasma cell leukemia-derived line, Eur. J. Immunol. 12: 641.PubMedCrossRefGoogle Scholar
  10. Glassy, M. C, and Ferrone, S., 1982, Differential segregation patterns of human chromosomes in somatic cell hybrids constructed with human B lymphocytes and human melanoma cells, Cancer Res. 42: 3971.PubMedGoogle Scholar
  11. Glassy, M. C, and Handley, H. H., 1985, Monoclonal antibodies and enzyme immunofiltration, in: Biotechnology Handbook ( P. N. Cheremisinoff and R. P. Ouelette, eds.), Technomic Publishing, Lancaster, Pennsylvania, p. 420.Google Scholar
  12. Glassy, M. C, Handley, H., Seegmiller, J. E., and Royston, 1., 1981, A lymphoblastoid B cell line useful for generating immunoglobulin secreting human—human hybridomas, Fed. Proc. 40: 996.Google Scholar
  13. Glassy, M. C, Handley, H. H., Hagiwara, H., and Royston, I., 1983a, UC 729–6, a human lymphoblastoid B cell line useful for generating antibody-secreting human—human hybridomas, Proc. Natl. Acad. Sci. USA 80: 6327.PubMedCrossRefGoogle Scholar
  14. Glassy, M. C, Handley, H. H., Cleveland, P. H., and Royston, I., 1983b, An enzyme immunofiltra- tion assay useful for detecting human monoclonal antibody, J. Immunol. Meth. 58: 119.CrossRefGoogle Scholar
  15. Glassy, M. C, Handley, H. H., Royston, I., and Lowe, D. H., 1983c, Human monoclonal antibodies to human cancers, in: Monoclonal Antibodies and Cancer ( B. D. Boss, R. E. Langman, I. S. Trombridge, and R. Dulbecco, eds.), Academic Press, New York, p. 163.Google Scholar
  16. Handley, H. H., and Royston, I., 1982, A human lymphoblastoid B cell line useful for generating immunoglobulin-secreting human hybridomas, in: Hybridomas in Cancer Diagnosis and Treatment ( M. S. Mitchell and H. F. Oettgen, eds.) Raven Press, New York, p. 125.Google Scholar
  17. Handley, H. H., Royston, I., and Glassy, M. C., 1983. The production of human monoclonal antibodies to human tumor associated antigens, in: Intercellular Communication in Leukocyte Function, Wiley-Interscience, New York, p. 617.Google Scholar
  18. Hellstrom, K. E., and Brown, J. P. 1979, Tumor antigens, in: The Antigens, Volume 5 ( M. Sela, ed.), Academic Press, New York, p. 1.Google Scholar
  19. Houghton, A. N., Brooks, H., Cote, R. J., Taormina, M. C., Oettgen, H. F., and Old, L. J., 1983, Detection of cell surface and intracellular antigens by human monoclonal antibodies. Hybrid cell lines derived from lymphocytes of patients with malignant melanoma, J. Exp. Med. 158: 53.PubMedCrossRefGoogle Scholar
  20. Köhler, G., and Milstein, C., 1975, Continuous cultures of fused cells secreting antibody of predefined specificity, Nature 256: 495.PubMedCrossRefGoogle Scholar
  21. Lever, J. E., Nuki, G., and Seegmiller, J. E., 1974, Expression of purine overproduction in a series of 8-azaguanine-resistant diploid human lymphoblast lines, Proc. Natl. Acad. Sci. USA 71: 2679.PubMedCrossRefGoogle Scholar
  22. Levy, J. A., Virolainen, M., and Defendi, V., 1968, Human lymphoblastoid lines from lymph node and spleen, Cancer 22: 517.PubMedCrossRefGoogle Scholar
  23. Levy, J. A., Buell, D. N., Creech, C., Hirshaut, Y., and Silverberg, H., 1971, Further charac- teristics of the WIL-1 and WIL-2 lymphoblastoid lines, J. Natl. Cancer Inst. 46:647PubMedGoogle Scholar
  24. Lowe, D. H., Handley, H. H., Schmidt, J., Royston, I., and Glassy, M. C, 1984, A human monoclonal antibody reactive with human prostate, J. Urol. 132:780.PubMedGoogle Scholar
  25. Olssen, L., and Kaplan, H. S., 1980, Human—human hybridomas producing monoclonal anti- bodies of predefined antigenic specificity, Proc. Natl. Acad. Sci. USA 77:5429.CrossRefGoogle Scholar
  26. Ruddle, F. H., 1973, Linkage analysis in man by somatic cell genetics, Nature 242:165.Google Scholar
  27. Schlom, J., Wunderlich, D., and Teramoto, Y. A., 1980, Generation of human monoclonalGoogle Scholar
  28. antibodies reactive with human mammary carcinoma cells. Proc. Natl. Acad. Sci. USA 77:6841. Sikora, K., and Phillips, J., 1981, Human monoclonal antibodies to glioma cells, Br. J. Cancer 43:105.Google Scholar
  29. Sikora, K., and Wright, R., 1981, Human monoclonal antibodies to lung-cancer antigens, Br. J. Cancer 43: 696.PubMedCrossRefGoogle Scholar
  30. Taylor, I. W., 1980, A rapid single step staining technique for DNA analysis by flow microfluorimetry, J. Histochem. Cytochem. 28: 1021.PubMedCrossRefGoogle Scholar
  31. Ueda, R., Shiku, H., Pfreundschuh, M., Takahashi, T., Li, L. T. C., Whitmore, Jr., W., Oettgen, H. F., and Old, L. J., 1979, Cell surface antigens of human renal cancer defined by autologous typing, J. Exp. Med. 150: 564.PubMedCrossRefGoogle Scholar
  32. Webb, K. S., Ware, J. L., Parks, S. F., and Paulson, D. F., 1981, A serologic approach to the definition of human prostatic carcinoma antigens, Prostate 2: 369.PubMedCrossRefGoogle Scholar

Copyright information

© Plenum Press, New York 1985

Authors and Affiliations

  • Mark C. Glassy
    • 1
    • 2
  • Harold H. Handley
    • 1
    • 2
  • Ivor Royston
    • 1
    • 2
  1. 1.Cancer CenterUniversity of CaliforniaSan DiegoUSA
  2. 2.Veterans Administration Medical CenterSan DiegoUSA

Personalised recommendations