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Formation and Role of Lipoxygenase Products in Human Platelets

  • M. Lagarde
  • M. Croset
  • M. Dechavanne
  • S. Renaud
  • K. S. Authi
  • N. Crawford
Part of the GWUMC Department of Biochemistry Annual Spring Symposia book series (GWUN)

Abstract

Blood platelets are involved in primary hemostasis through their aggregation in response to different agents. When platelets are aggregated by some of these agents such as thrombin or collagen, polyunsaturated fatty acids (mainly arachidonic acid) are liberated from membrane phospholipids and then subsequently oxygenated via cyclooxygenase and/or lipoxygenase. The cyclooxygenase pathway has been well documented (Smith, 1980). From arachidonic acid (AA), it leads to proaggregatory prostanoids, prostaglandin endoperoxides (PGG2/PGH2) and thromboxane A2 (TxA2), which together with 12-hydroxyheptadecatrienoic acid represent the main compounds of the pathway, primary prostaglandins being minor products. In comparison, dihomo-γ-linolenic acid (DHLA) and 5,8,11,14,17-eicosapentaenoic acid (EPA), precursors of mono- and trienoic series prostanoids, respectively, are well known to be inhibitors of platelet functions (Willis, 1981). Less data about platelet lipoxygenase pathway, especially for its biological role, are available. This enzyme is assumed to be a 12-lipoxygenase (Hamberg et ai., 1974; Nugteren, 1975) associated with a glutathione-dependent peroxidase (Bryant and Bailey, 1980). It has been reported that 12-hydroperoxyeicosatetraenoic acid (12-HPETE), the lipoxygenase product of AA, is able to inhibit both thromboxane synthase (Hammarström and Falardeau, 1977) and platelet diglyceride lipase (Rittenhouse-Simmons, 1980). More recently, 12-HPETE was described as an inhibitor of PGH2/TxA2-induced platelet aggregation (Aharony et al., 1982).

Keywords

Arachidonic Acid Platelet Aggregation Human Platelet Lipoxygenase Activity Lipoxygenase Pathway 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer Science+Business Media New York 1985

Authors and Affiliations

  • M. Lagarde
    • 1
  • M. Croset
    • 1
  • M. Dechavanne
    • 1
  • S. Renaud
    • 1
  • K. S. Authi
    • 2
  • N. Crawford
    • 2
  1. 1.Institut Pasteur, Laboratoire d’Hémobiologie, Faculté Alexis CarrelInserm U 63Lyon Cédex 08France
  2. 2.Department of BiochemistryRoyal College of SurgeonsLondonUK

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