Prostaglandin Endoperoxide Synthase from Human Cell Line Lu-65
In this chapter, we present our data on the isolation and properties of prostaglandin endoperoxide synthase (PES; E.C. 184.108.40.206) from a human cell line. This enzyme has been isolated and purified to homogeneity in other species; partially purified human platelet PES that retained activity has been prepared by DEAE-chromatog-raphy (Hammarström and Falardeau, 1977) and has been purified to near-homogeneity (Ho et al., 1980). In other studies, an inactive human platelet PES has been identified by SDS-PAGE (Roth and Majerus, 1975). Preliminary data on the characterization of this PES from a human cell line, Lu-65, is presented.
KeywordsArachidonic Acid Prostaglandin Synthetase Cyclooxygenase Activity Eicosatrienoic Acid Prostaglandin Endoperoxide
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