Advertisement

Release of Leukotrienes B4 and D4 by the Anaphylactic Guinea Pig Lung in Vitro

  • Hassan Salari
  • Pierre Borgeat
  • Pierre Braquet
  • Yvon Brousseau
  • Pierre Sirois
Part of the GWUMC Department of Biochemistry Annual Spring Symposia book series (GWUN)

Abstract

The lung possesses several enzymic systems for the metabolism of arachidonic acid (Samuelsson et al., 1975; Hammarström, 1983). Among these, the 5-lipoxygenase has recently received considerable attention since some of its products, the leukotrienes (LTs), may play critical roles in inflammation (Samuelsson, 1982; Lewis and Austen, 1981) and immediate hypersensitivity reactions (Holroyde et al., 1981; Dahlén et al., 1980). Indeed, leukotriene B4 (LTB4) was shown to be a potent chemotactic substance towards polymorphonuclear leukocytes (PMNL) (Ford-Hutchison et al., 1980), and leukotrienes C4 (LTC4), D4 (LTD4), and E4 (LTE4) were found to have strong myotropic activity on smooth muscles of the respiratory tract (Drazen et al., 1980; Hedqvist et al., 1980; Lewis et al., 1980).

Keywords

Lung Perfusate Antigen Challenge Eicosatetraenoic Acid Absorbance Unit Full Scale Mouse Mastocytoma Cell 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Borgeat, P., Fruteau de Laclos, B., Rabinovitch, H., Picard, S., Braquet, P., Hebert, J., and Laviolette, M., 1984, Eosinophil-rich human polymorphonuclear leukocyte preparations characteristically release leukotriene C4 upon ionophore A23187 challenge, J. Allergy Clin. Immunol. 74:310–315.PubMedCrossRefGoogle Scholar
  2. Brocklehurst, W.E., 1960, The release of histamine and formation of a slow-reacting substance (SRS-A) during anaphylactic shock, J. Physiol. (Lond.) 151:416–435.Google Scholar
  3. Dahlén, S.E., Hedqvist, P., Hammarström, S., and Samuelsson, B., 1980, Leukotrienes are potent constrictors of human bronchi, Nature 288:484–486.PubMedCrossRefGoogle Scholar
  4. Drazen, J.M., Austen, K.F., Lewis, R.A., Clark, D.A., Goto, G., Marfat, A., and Corey, E.J., 1980, Comparative airway and vascular activities of leukotrienes C-l and D in vivo and in vitro, Proc. Natl. Acad. Sci. U.S.A. 77:4354–4358.CrossRefGoogle Scholar
  5. Engineer, D.M., Niederhauser, U., Piper, P.J., and Sirois, P., 1978, Release of mediators of ana-phyIaxis: Inhibition of prostaglandin synthesis and the modification of release of slow-reacting substances of anaphylaxis and histamine, Br. J. Pharmacol. 62:61–66.PubMedCrossRefGoogle Scholar
  6. Fleisch, J.H., Haisch, K.D., and Spaethe, S.M., 1982, Slow reacting substance of anaphylaxis (SRS-A) release from guinea-pig lung parenchyma during antigen or ionophore-induced contraction, J. Pharmacol. Exp. Ther. 221:146–151.PubMedGoogle Scholar
  7. Ford-Hutchison, A.W., Bray, M.A., Doig, M.V., Shipley, M.E., and Smith, M.J.H., 1980, Leukotriene B, a potent chemokinetic and aggregating substance released from polymorphonuclear leukocytes, Nature 286:264–265.CrossRefGoogle Scholar
  8. Hammarström, S., 1983, Leukotrienes, Annu. Rev. Biochem. 52:355–377.PubMedCrossRefGoogle Scholar
  9. Hansson, G., Björck, T., Dahlén, S.-E., Hedqvist, P., Granström, E., and Dahlén, B., 1983, Specific allergen induces contraction of bronchi and formation of leukotrienes C4, D4 and E4 in human asthmatic lung, Adv. Prostaglandin Thromboxane Leukotriene Res. 12:153–159.Google Scholar
  10. Hedqvist, P., Dahlén, S.-E., Gustafsson, L., Hammarström, S., and Samuelsson, B., 1980, Biological profile of leukotrienes C4 and D4, Acta Physiol. Scand. 110:331–333.PubMedCrossRefGoogle Scholar
  11. Holroyde, M.C, Altounyan, R.E.C, Cole, M., Dixon, M., and Elliott, E.V., 1981, Bronchocon-striction produced in man by leukotrienes C and D, Lancet 2:17–18.PubMedCrossRefGoogle Scholar
  12. Kellaway, C.H., and Trethewie, E.R., 1940, The liberation of a slow-reacting smooth-muscle stimulating substance in anaphylaxis, Q. J. Exp. Physiol. 30:121–145.Google Scholar
  13. Lewis, R.A., and Austen, K.F., 1981, Mediation of local homeostasis and inflammation by leukotrienes and other mast cell-dependent compounds, Nature 293:103–108.PubMedCrossRefGoogle Scholar
  14. Lewis, R.A., Austen, K.F., Drazen, J.M., Clark, D.A., Marfat, A., and Corey, E.J., 1980, Slow reacting substances of anaphylaxis: Identification of leukotrienes C-l and D from human and rat sources, Proc. Natl. Acad. Sci. U.S.A. 77:3710–3714.PubMedCrossRefGoogle Scholar
  15. Morris, H. R., Taylor, G. W., Piper, P. J., and Tippins, J. R., 1979, Slow reacting substance of anaphylaxis. Studies on purification and characterization. Agents Actions 5(Suppl. 6):27–36.Google Scholar
  16. Morris, H.R., Taylor, G.W., Piper, P.J., and Tippins, J.R., 1980a, Structure of slow-reacting substance of anaphylaxis from guinea-pig lung, Nature 285:104–106.PubMedCrossRefGoogle Scholar
  17. Morris, H.R., Taylor, G.W., Piper, P.J., Samhoun, M.N., and Tippins, J.R., 1980b, Slow-reacting substances (SRSs): The structure identification of SRSs from rat basophilic leukemia (RBL-1) cells, Prostaglandins 19:185–201.PubMedCrossRefGoogle Scholar
  18. Murphy, R.C, Hammarström, S., and Samuelsson, B., 1979, Leukotriene C, a slow reacting substance (SRS) from mouse mastocytoma cells, Proc. Natl. Acad. Sci. U.S.A. 79:4275–279.CrossRefGoogle Scholar
  19. Samuelsson, B., 1982, Leukotrienes: A new group of biologically active compounds, in: Advances in Pharmacology and Therapeutics II, Vol. 4, Biochemical and Immunological Pharmacology (H. Yoshida, Y. Hagihara, and S. Ebashi, eds.), Permagon Press, Oxford, New York, pp. 55–75.Google Scholar
  20. Samuelsson, B., Granström, E., Green, K., Hamberg, M., and Hammarström, S., 1975, Prostaglandins, Rev. Biochem. 44:669–695.CrossRefGoogle Scholar
  21. Weichman, B.M., Hostelley, L.S., Bostick, S.P. Muccitelli, R.M., Krell, R.D., and Gleason, J.G., 1982, Regulation of the synthesis and release of slow-reacting substance of anaphylaxis from sensitized monkey lung, J. Pharmacol. Exp. Ther. 221:295–302.PubMedGoogle Scholar

Copyright information

© Springer Science+Business Media New York 1985

Authors and Affiliations

  • Hassan Salari
    • 1
  • Pierre Borgeat
    • 1
  • Pierre Braquet
    • 2
  • Yvon Brousseau
    • 3
  • Pierre Sirois
    • 3
  1. 1.Groupe de Recherche sur les Leucotriènes, Laboratoire d’Endocrinologie MoléculaireCentre Hospitalier de l’Université LavalQuébecCanada
  2. 2.I. H. B. Research LaboratoriesLe Plessis-RobinsonFrance
  3. 3.Département de PédiatrieCentre Hospitalier Universitaire, Université de SherbrookeSherbrookeCanada

Personalised recommendations