Stimulation of Lymphocyte Guanylate Cyclase by Arachidonic Acid and HETEs
Mitogen activation of human peripheral blood lymphocytes (HPBL) occurs as a consequence of several early membrane events. One of the earliest is the Ca2+ -dependent stimulation of guanylate cyclase (Coffey et al., 1981). Experiments with the T-cell mitogens phytohemagglutinin (PHA), concanavalin A (Con A), and phorbol myristate acetate (PMA) suggested that phospholipase and lipoxygenase activation are important in the sequence of early membrane events leading to guanylate cyclase stimulation, cGMP accumulation, and DNA synthesis (Coffey and Hadden,1981, 1983a,b). Cyclooxygenase (Coffey et al., 1981) and thromboxane synthetase activities (Gordon et al., 1981) were not found to be essential for mitogenesis.
KeywordsPhorbol Myristate Acetate Guanylate Cyclase Mitogen Activation Phorbol Myristate Acetate Soluble Guanylate Cyclase
Unable to display preview. Download preview PDF.
- Coffey, R. G., and Hadden, J. W., 1981, Arachidonate and metabolites in mitogen activation of lymphocyte guanylate cyclase, in: Advances in Immunopharmacology (J. Hadden, L. Chedid, P. Mullen, and F. Spreafico, eds.), Pergamon Press, Oxford, pp. 365–373.Google Scholar
- Coffey, R. G., and Hadden, J. W., 1983a, Calcium and guanylate cyclase in lymphocyte activation, in: Advances in Immunopharmacology, Vol. 2 (J. W. Hadden, L. Chedid, P. Dukor, F. Spreafico, and D. Willoughby, eds.), Pergamon Press, Oxford, pp. 87–94.Google Scholar
- White, A.A., Karr, D.B., and Patt, C.S., 1982, Role of lipoxygenase in the O2-dependent activation of soluble guanylate cyclase from rat lung, Biochem. J. 204:382–392.Google Scholar