Stimulation of Lymphocyte Guanylate Cyclase by Arachidonic Acid and HETEs

  • Ronald G. Coffey
  • John W. Hadden
Part of the GWUMC Department of Biochemistry Annual Spring Symposia book series (GWUN)


Mitogen activation of human peripheral blood lymphocytes (HPBL) occurs as a consequence of several early membrane events. One of the earliest is the Ca2+ -dependent stimulation of guanylate cyclase (Coffey et al., 1981). Experiments with the T-cell mitogens phytohemagglutinin (PHA), concanavalin A (Con A), and phorbol myristate acetate (PMA) suggested that phospholipase and lipoxygenase activation are important in the sequence of early membrane events leading to guanylate cyclase stimulation, cGMP accumulation, and DNA synthesis (Coffey and Hadden,1981, 1983a,b). Cyclooxygenase (Coffey et al., 1981) and thromboxane synthetase activities (Gordon et al., 1981) were not found to be essential for mitogenesis.


Phorbol Myristate Acetate Guanylate Cyclase Mitogen Activation Phorbol Myristate Acetate Soluble Guanylate Cyclase 


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Copyright information

© Springer Science+Business Media New York 1985

Authors and Affiliations

  • Ronald G. Coffey
    • 1
  • John W. Hadden
    • 1
  1. 1.Program of ImmunopharmacologyUniversity of South Florida Medical CollegeTampaUSA

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