Membrane Events and Guanylate Cyclase Activation in Mitogen-Stimulated Lymphocytes
The lymphocyte has provided an important model for the study of the early biochemical events associated with the initiation of cellular proliferation. Resting lymphocytes (G0 or restricted G1 phase), as obtained from human peripheral blood, can be induced in large numbers to undergo clonal proliferation using a variety of mitogens. The two most commonly employed plant lectin mitogens are phytohem-agglutinin (PHA) and concanavalin A (Con A). The initiation process is characterized by rapid, saturable binding of the lectin to cellular receptors (<30 min), followed by a series of cellular events involving probably three cell types.
KeywordsLymphocyte Proliferation Cyclic Nucleotide Guanylate Cyclase Lymphocyte Activation Phorbol Myristate Acetate
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