A Two-Receptor Model for the Mechanism of Action of Prostaglandins in the Renal Collecting Tubule

  • William L. Smith
  • Arlyn Garcia-Perez
Part of the GWUMC Department of Biochemistry Annual Spring Symposia book series (GWUN)


Studies on the mechanism of action of steroid hormones have led to the concept that different steroids act through different receptors present in different target cells to elicit a common biochemical response, namely, regulation of transcription. One might presume that different prostaglandins also operate through different receptors to cause some common response. However, this response is still not defined. In this chapter we summarize our recent studies on the metabolism and function of prostaglandins by the renal collecting tubule (Garcia-Perez and Smith, 1983, 1984). Our major focus is to describe the development of a two-receptor model for the actions of prostaglandins in these cells.


Adenylate Cyclase Tubule Cell Basolateral Side Basolateral Surface Thin Limb 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. Anderson, R.J., Berl, T., McDonald, K.M., and Schrier, R.W., 1975, Evidence for an in vivo antagonism between vasopressin and prostaglandin in the mammalian kidney, J. Clin. Invest. 56:420–426.PubMedCrossRefGoogle Scholar
  2. Currie, M.G., and Needleman, P., 1982, Distribution of prostaglandin synthesis along the rabbit nephron, Fed. Proc. 41:1545.Google Scholar
  3. Fejes-Toth, G. A., Magyar, A., and Walter, J., 1977, Renal response to vasopressin after inhibition of prostaglandin synthesis, Am. J. Physiol. 232:F416–F423.PubMedGoogle Scholar
  4. Garcia-Perez, A., and Smith, W. L., 1983, Use of monoclonal antibodies to isolate cortical collecting tubule cells: AVP induces PGE release, Am. J. Physiol. 244:C211–C220.PubMedGoogle Scholar
  5. Garcia-Perez, A., and Smith, W.L., 1984, Apical-basolateral membrane asymmetry in canine cortical collecting tubule cells: Bradykinin, arginine vasopressin, prostaglandin E2 interrelationships, J. Clin. Invest. 74:63–74.PubMedCrossRefGoogle Scholar
  6. Garrity, M.J., Andreason, T.J., Storm, D.R., and Robertson, R.P., 1983, Prostaglandin E-induced heterologous desensitization of hepatic adenylate cyclase: Consequences on the guanyl nucleotide regulatory complex, J. Biol. Chem. 258:8692–8697.PubMedGoogle Scholar
  7. Grantham, J.J., and Orloff, J., 1968, Effect of prostaglandin E1 on the permeability response of the isolated collecting tubule to vasopressin, adenosine 3′,5′-monophosphate and theophylline, J. Clin. Invest. 47:1154–1161.PubMedCrossRefGoogle Scholar
  8. Handler, J.S., and Orloff, J., 1981, Antidiuretic hormone, Annu. Rev. Physiol. 43:611–624.PubMedCrossRefGoogle Scholar
  9. Hansen, H.S., 1981, Essential fatty acid-supplemented diet increases renal excretion of prostaglandin E2 and water in essential fatty acid-deficient rats, Lipids 16:849–854.PubMedCrossRefGoogle Scholar
  10. Hassid, A., 1982, Regulation of prostaglandin biosynthesis in cultured cells, Am. J. Physiol. 243:C205–C211.PubMedGoogle Scholar
  11. Hopkins, N.K., and Gorman, R.R., 1981, Regulation of endothelial cell cyclic nucleotide metabolism by prostacyclin, J. Clin. Invest. 67:540–546.PubMedCrossRefGoogle Scholar
  12. Kassis, S., and Fishman, P.H., 1982, Different mechanisms of desensitization of adenylate cyclase by isoproterenol and prostaglandin E1 in human fibroblasts, J. Biol. Chem. 257:5312–5381.PubMedGoogle Scholar
  13. Lum, G.M., Aisenberg, G.A., Dunn, M.J., Berl, T., Schrier, R.W., and McDonald, K.M., 1977, In vivo effect of indomethacin to potentiate the renal medullary cyclic AMP response to vasopressin, J. Clin. Invest. 59:8–13.PubMedCrossRefGoogle Scholar
  14. Minkes, M., Stanford, N., Chi. M.-Y., Roth, G.J., Raz, A., Needleman, P., and Majerus, P.W., 1977, Cyclic adenosine 3′,5′-monophosphate inhibits the availability of arachidonate to prostaglandin synthetase in human platelet suspensions, J. Clin. Invest. 59:449–454.PubMedCrossRefGoogle Scholar
  15. Smith, W. L., and Bell, T. G., 1978, Immunohistochemical localization of the prostaglandin-forming cyclooxygenase in renal cortex, Am. J. Physiol. 235:F451–F457.PubMedGoogle Scholar
  16. Smith, W.L., Bell, T.G., and Needleman, P., 1979, Increased renal tubular synthesis of prostaglandins in the rabbit kidney in response to ureteral obstruction, Prostaglandins 18:269–277.PubMedCrossRefGoogle Scholar
  17. Smith, W. L., Grenier, F. C, DeWitt, D. L., Garcia-Perez, A., and Bell, T. G., 1983, Cellular compartmentalization of the biosynthesis and function of PGE2 and PGI2 in the renal medulla, in: Prostaglandins and the Kidney (M. J. Dunn, C. Patrons, and G. A. Cinotti, eds.), Plenum Press, New York, pp. 27–39.CrossRefGoogle Scholar
  18. Torikai, S., and Kurokawa, K., 1981, Distribution of prostaglandin E2-sensitive adenylate cyclase along the rat nephron, Prostaglandins 21:427–438.PubMedCrossRefGoogle Scholar
  19. Torikai, S., and Kurokawa, K., 1983, Effect of PGE2 on vasopressin-dependent cell cAMP in isolated single nephron segments, Am. J. Physiol. 245: F58–F66.PubMedGoogle Scholar

Copyright information

© Springer Science+Business Media New York 1985

Authors and Affiliations

  • William L. Smith
    • 1
  • Arlyn Garcia-Perez
    • 1
  1. 1.Department of BiochemistryMichigan State UniversityEast LansingUSA

Personalised recommendations