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Quantitation of Arachidonate Released during the Platelet Phosphatidylinositol Response to Thrombin

  • J. Bryan Smith
  • Carol Dangelmaier
  • Gerard Mauco
Part of the GWUMC Department of Biochemistry Annual Spring Symposia book series (GWUN)

Abstract

Several workers have shown that decreases in the radioactivity of phosphatidylinositol (PI) and phosphatidylcholine (PC) occur when platelets prelabeled with radioactive arachidonic acid are treated with thrombin (see Rittenhouse-Simmons and Deykin, 1981, for review). These decreases have been attributed to arachidonate release by one of two mechanisms (Fig. 1). In one mechanism, PI is hydrolyzed by a Pi-specific phospholipase C, yielding diacylglycerol and inositol phosphate. The diacylglycerol is further hydrolyzed by a lipase or lipases, releasing arachidonic acid and other fatty acids. In the other mechanism, PC is hydrolyzed by a phospholipase A2, releasing arachidonic acid and other fatty acids from the 2-position and producing lysophosphatidylcholine (LPC). There is also some evidence that arachidonic acid is made available by the action of phospholipase A2 on phospha-tidylethanolamine.

Keywords

Arachidonic Acid Phosphatidic Acid Phosphatidic Acid Inositol Phosphate Heptadecanoic Acid 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. Broekman, M.J., Ward, J.W., and Marcus, A.J., 1980, Phospholipid metabolism in stimulated human platelets. Changes in phosphatidylinositol, phosphatidic acid and lysophospholipids, J. Clin. Invest. 66:275–283.PubMedCrossRefGoogle Scholar
  2. Broekman, M.J., Ward, J.W., and Marcus, A.J., 1981, Fatty acid composition of phosphatidylinositol and phosphatidic acid in stimulated platelets. Persistence of arachidonyl-stearyl structure, J. Biol. Chem. 256:8271–8274.PubMedGoogle Scholar
  3. McKean, M.L., Smith, J.B., and Silver, M.J., 1981, Formation of lysophosphatidylcholine by human platelets in response to thrombin, J. Biol. Chem. 256:1522–1524.PubMedGoogle Scholar
  4. Rittenhouse-Simmons, S., and Deykin, D., 1981, Release and metabolism of arachidonate in human platelets, in: Platelets in Biology and Pathology (J. Gordon, ed.), Elsevier/North-Holland Biomedical Press, Amsterdam, pp. 349–372.Google Scholar

Copyright information

© Springer Science+Business Media New York 1985

Authors and Affiliations

  • J. Bryan Smith
    • 1
  • Carol Dangelmaier
    • 1
  • Gerard Mauco
    • 1
  1. 1.Center for Thrombosis ResearchTemple Medical SchoolPhiladelphiaUSA

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