Occurrence of Leukotrienes in Rat Brain
Leukotrienes (LT) are bioactive compounds with proposed roles as mediators of inflammation and allergy (Samuelsson, 1983). Production of LT is initiated by 5-lipoxygenation of arachidonic acid to 5(S)-hydroperoxyeicosatetraenoic acid (5-HPETE), which is further transformed to an unstable epoxide (LTA4) (Borgeat et al., 1976; Borgeat and Samuelsson, 1979a). This intermediate can be enzymatically hydrolyzed to LTB4 (Borgeat and Samuelsson, 1979b; Corey et al., 1981). Alter-natively, LTA4 is converted, by addition of glutathione at C-6, into LTC4 (Murphy et al., 1979; Morris et al., 1980). Stepwise enzymatic elimination of glutamic acid and glycine in the peptide side chain leads to formation of LTD4 and LTE4, respectively (Örning and Hammarström, 1980; Bernström and Hammarström, 1980). The proposed mediator of allergic reactions, slow-reacting substance of anaphylaxis (SRS-A) (Orange and Austen, 1969), has been identified as an entity composed of LTC4, LTD4, and LTE4 (Samuelsson, 1983).
KeywordsHPLC Glutathione Chloroform Toluene Glycine
Unable to display preview. Download preview PDF.
- Andersson, K., Nielsen, O.G., Toftgård, R., Eneroth, P., Gustavsson, J.-Å., Battistini, N., and Agnati, L.S., 1983, Increased aminé turnover in several hypothalamic noradrenaline nerve terminal systems and changes in prolactic secretion in the male rat by exposure to various concentrations of toluene, NeurotoxicologyNeurotoxicology 4(4):43–56.Google Scholar
- Björck, T., Dahlén, S.-E., Gustavsson, L.-E., and Hedqvist, P., 1985, Characterization of the contractile responses to leukotrienes C4, D4, and E4 in the guinea pigileum, Ada Physiol. Scand. (in press).Google Scholar
- Dahlén, S.-E., 1983, Pulmonary effects of leukotrienes, Ada Physiol. Scand. 118(Suppl. 512):1–51.Google Scholar
- Hulting, A. L., Lindgren, J. Å., Hökfelt, T., Eneroth, P., Werner, S., and Samuelsson, B., 1985, Leukotriene C4 as a mediator of LH release from rat anterior pituitary cells, Nature Proc. Natl. Acad. Sci. U.S.A.(in press).Google Scholar