Human Trabecular Meshwork Cells

Arachidonic Acid Metabolism and Prostaglandin Release
  • Mary E. Gerritsen
  • Bernard I. Weinstein
  • Gary G. Gordon
  • A. Louis Southren
Part of the GWUMC Department of Biochemistry Annual Spring Symposia book series (GWUN)


In man, most of the aqueous humor drainage and the major resistance to outflow occur across the trabecular meshwork and inner wall of Schlemm’s canal. Pros-taglandins are known to alter intraocular presure (IOP) and have been implicated in both physiological and pathophysiological aspects of IOP regulation. In the present study, we have characterized pathways of arachidonic acid metabolism in human trabecular meshwork (HTM) cells and measured basal and hormone-stimulated PGE2 release. The principal product of [1-14C]PGH2 metabolism and [1 − 14C]arachidonic acid metabolism was PGE2. Immunoreactive PGE2 was released in excess of immunoreactive 6-keto-PGF during 15-min incubations in phosphate-buffered saline. Incubation wth indomethacin (5 μM) for 20 min substantially reduced (80–85% inhibition) PGE2 formation from AA. Overnight incubation with dexamethasone (ID50 = 3.3 × 10−9M) inhibited basal and A23187-stimulated PGE2 release. The effects of dexamethasone on PGE2 synthesis and release could be prevented by pretreatment of the HTM cells with 2 μg/ml cyclo-heximide. A23187 (10−6-10−5 M) elicited a significant P <0.05 compared to control) increase in PGE2 release (RIA determinations).


Trabecular Meshwork Arachidonic Acid Metabolism PGE2 Synthesis Primary Open Angle Glaucoma PGE2 Release 
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Copyright information

© Springer Science+Business Media New York 1985

Authors and Affiliations

  • Mary E. Gerritsen
    • 1
  • Bernard I. Weinstein
    • 2
  • Gary G. Gordon
    • 2
  • A. Louis Southren
    • 2
  1. 1.Department of PhysiologyNew York Medical CollegeValhallaUSA
  2. 2.Departments of Ophthalmology and MedicineNew York Medical CollegeValhallaUSA

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