Regulation of Cyclooxygenase Synthesis in Vascular Smooth Muscle Cells by Epidermal Growth Factor
The prostaglandin synthases, which are widely distributed throughout the various organ systems of the body, are activated primarily by release of substrate arachidonic acid from cellular phospholipids (Hamberg et al., 1975). These prostaglandin-synthesizing systems may become refractory to further challenge by arachidonic acid substrate because of a self-inactivating feature of the cyclooxygenase enzyme first characterized by Smith and Lands (1972). This is mediated by free-radical intermediates of the reaction, which permanently inactivate the enzyme. An analogous refractoriness also develops following exposure to aspirin (Vane, 1971) and is caused by acetylation of a serine residue in the active site (Roth et al., 1975).
KeywordsEpidermal Growth Factor Arachidonic Acid Vascular Smooth Muscle Cell Confluent Culture Aspirin Treatment
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