The Major Pathway for Leukotriene B4 Catabolism in Human Polymorphonuclear Leukocytes Involves ω-Oxidation by a Cytochrome P-450 Enzyme

  • Steven Shak
  • Ira M. Goldstein
Part of the GWUMC Department of Biochemistry Annual Spring Symposia book series (GWUN)


Leukotriene B4 (LTB4), or 5(S),12(R)-dihydroxy-6,14-cis-8,10-trans-eicosatetrae-noic acid, is a product of the 5-lipoxygenase pathway in human polymorphonuclear leukocytes (PMN) and has received a great deal of attention recently because of the role that it may play as a mediator of inflammation (Samuelsson, 1983). Leukotriene B4 acts in vitro to provoke directed migration (chemotaxis) as well as enhanced random migration (chemokinesis) of human PMN (Goetzl and Pickett, 1980). It also provokes selective release of granule-associated (lysosomal) enzymes from cytochalasin-B-treated PMN (Goetzl and Pickett, 1980; Feinmark et al., 1981) and causes these cells to aggregate (O’Flaherty et al., 1981). In vivo, LTB4 induces adherence of PMN to the walls of postcapillary venules in the hamster cheek pouch (Dahlen et al., 1981), PMN-dependent increases in vascular permeability in rabbit skin (Wedmore and Williams, 1981), and exudation of PMN in human skin (Soter et al., 1983).


Phorbol Myristate Acetate Chronic Granulomatous Disease Phorbol Myristate Acetate Mononuclear Leukocyte Methyl Formate 


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Copyright information

© Springer Science+Business Media New York 1985

Authors and Affiliations

  • Steven Shak
    • 1
    • 2
  • Ira M. Goldstein
    • 1
    • 2
  1. 1.Cardiovascular Research Institute and Rosalind Russell Arthritis Research Laboratory, Department of MedicineUniversity of CaliforniaSan FranciscoUSA
  2. 2.Medical ServiceSan Francisco General HospitalSan FranciscoUSA

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