The Major Pathway for Leukotriene B4 Catabolism in Human Polymorphonuclear Leukocytes Involves ω-Oxidation by a Cytochrome P-450 Enzyme
Leukotriene B4 (LTB4), or 5(S),12(R)-dihydroxy-6,14-cis-8,10-trans-eicosatetrae-noic acid, is a product of the 5-lipoxygenase pathway in human polymorphonuclear leukocytes (PMN) and has received a great deal of attention recently because of the role that it may play as a mediator of inflammation (Samuelsson, 1983). Leukotriene B4 acts in vitro to provoke directed migration (chemotaxis) as well as enhanced random migration (chemokinesis) of human PMN (Goetzl and Pickett, 1980). It also provokes selective release of granule-associated (lysosomal) enzymes from cytochalasin-B-treated PMN (Goetzl and Pickett, 1980; Feinmark et al., 1981) and causes these cells to aggregate (O’Flaherty et al., 1981). In vivo, LTB4 induces adherence of PMN to the walls of postcapillary venules in the hamster cheek pouch (Dahlen et al., 1981), PMN-dependent increases in vascular permeability in rabbit skin (Wedmore and Williams, 1981), and exudation of PMN in human skin (Soter et al., 1983).
KeywordsPhorbol Myristate Acetate Chronic Granulomatous Disease Phorbol Myristate Acetate Mononuclear Leukocyte Methyl Formate
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- Boyum, A., 1968, Isolation of mononuclear cells and granulocytes from human blood. Isolation of monocuclear cells by one centrifugation and of granulocytes of combining centrifugation and sedimentation at 1g, Scand. J. Clin. Lab. Invest. 21(Suppl. 97):77–89.Google Scholar
- Dahlén, S.-E., Bjork, J., Hedqvist, P., Arfors, K.-E., Hammarström, S., Lindgren, J.A., and Samuelsson, B., 1981, Leukotrienes promote plasma leakage and leukocyte adhesion in postcapillary venules: In vivo effect with relevance to the acute inflammatory response, Proc. Natl. Acad. Sci. U.S.A. 78:3887–3891.PubMedCrossRefGoogle Scholar