In Vivo Quantification and Platelet Survival Studies: What is the Best Approach?

  • A. P. du Heyns
Part of the NATO ASI Series book series (NSSA, volume 88)


Although the role of blood platelets in health and disease may be assessed with in vitro methods such as platelet aggregometry, these have limited value and have been found wanting in the clinical situation, except in a few well-defined diseases. Recently investigators have relied increasingly on in vivo measurements of platelet behaviour. For many years the determination of platelet survival time was regarded as the best determinant of in vivo platelet behavior. It was also accepted that this was best done with sodium-dichromate as platelet label (1). In an important systematic investigation of alternative gamma-emitting radionuclides, Thakur(2) recommended In-111 as a suitable and preferable platelet label. The discovery of oxine and other chelates, has now established the superiority of In-111-complexes over Cr-51 as a platelet label.


Gamma Photon Platelet Deposition Scintillation Camera Platelet Survival Fitted Regression Curve 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    ISCH panel on diagnostic application of radioisotopes in hematology, Recommended methods for radioisotope platelet survival studies, Blood. 50: 1137 (1977).Google Scholar
  2. 2.
    M. L. Thakur, M. J. Welch, J. H. Joist, R. E. Coleman, Indium-111 labeled platelets: studies on preparation and evaluation of in vitro and in vivo functions, Thromb Res 9: 345 (1976).PubMedCrossRefGoogle Scholar
  3. 3.
    A. du P. Heyns, M. G. Lotter, P. N. Badenhorst, O. R. van Reenen, H. Pieters, P. C. Minnaar, F. P. Retief, Kinetics, distribution and sites of destruction of Indium-111-labeled human platelets, Br J Haematol 44: 269 (1980).CrossRefGoogle Scholar
  4. 4.
    J. H. Joist (Guest Editor), Indium labeled platelets, Sem Thromb Hemostas 9: 79 (1983).CrossRefGoogle Scholar
  5. 5.
    A. du P. Heyns, M. G. Lotter, P. N. Badenhorst, F. de Kock, H. Pieters, C. Herbst, O. R. van Reenen, H. Kotze, P. C. Minnaar, Kinetics and sites of destruction of Indium-111-oxine labeled platelets in idiopathic thrombocytopenic purpura: a quantitative study, Am J Hematol 12: 167 (1982).CrossRefGoogle Scholar
  6. 6.
    P. N. Badenhorst, M. G. Lotter, A. du P. Heyns, O.R. van Reenen, C. Herbst, H. Pieters, H. F. Kotze, L. J. Duyvene de Wit, P. C. Minnaar, The influence of the “collection injury” on the survival and distribution of Indium-111-labeled platelets, Br J Haematol 52: 233 (1982).PubMedCrossRefGoogle Scholar
  7. 7.
    E. A. Murphy and M. E. Francis, The estimation of blood platelet survival. H. The multiple hit model, Thromb Diath Haemorrh 25: 53 (1971).PubMedGoogle Scholar
  8. 8.
    A. C. Dornhorst, The interpretation of red cell survival curves, Blood 6: 1284 (1951).PubMedGoogle Scholar
  9. 9.
    T. L. Simon, T. M. Hyers, J. P. Gaston, L. A. Harker, Heparin pharmacokinetics: increased requirements in pulmonary embolism, Br J Haematol 39: 111 (1978).PubMedCrossRefGoogle Scholar
  10. 10.
    J. M. Paulus, Measuring mean life span, mean age, and variance of longevity in platelets, in: “Platelet Kinetics,” J. M. Paulus, ed. Amsterdam, North Holland (1971).Google Scholar
  11. 11.
    D. G. Meuleman, G. M. T. Vogel, S. M. Stulemeyer, H. C. T. Moelker, Analysis of platelet survival curves in an arterial thrombus model in rats, Thromb Res 20: 31 (1980).PubMedCrossRefGoogle Scholar
  12. 12.
    A. du P. Heyns, M. G. Lotter, H. F. Kotzke, H. Pieters, P. Wessels, Quantification of in vivo distribution of platelets labeled with Indium-111-oxine, J Nucl Med 23: 943 (1982)Google Scholar
  13. 13.
    A. du P. Heyns, M. G. Lotter, P. N. Badenhorst, Platelet imaging, in: “Methods in Haematology,” L. A. Harker, ed. Churchill Livingstone, London (1983).Google Scholar
  14. 14.
    W. J. Powers, C. J. Mathias, K. T. Hopkins, J. B. Rubin, B. A. Siegel, M. J. Welch, Dual radiotracer techniques for improved scintigraphic detection of thrombi, in: “Proceedings of the Third World Congress of Nuclear Medicine and Biology,” C. Raynaud, ed. Pergamon Press, Paris (1982).Google Scholar

Copyright information

© Plenum Press, New York 1985

Authors and Affiliations

  • A. P. du Heyns
    • 1
  1. 1.M.R.C. Blood Platelet Research UnitUniversity of the Orange Free StateBloemfonteinSouth Africa

Personalised recommendations