Cell Isolation Techniques: A Critical Review

  • M. R. Hardeman
Part of the NATO ASI Series book series (NSSA, volume 88)


Labeling blood cells with a radioactive tracer is not a new technique. In 1940 Hahn and Hevesy measured the blood volume of a rabbit by labeling the animal’s erythrocytes with radioactive phosphorus. Since then many experimental and clinical investigations using radioactive labeled cellular blood elements have been described in the literature. Their application has become increasingly popular during the past few years, due to the development of very efficient labeling techniques and the availability of suitable radionuclides. The following criteria need to be fulfilled by an ideal blood cell labeling method.


Immunol Method Centrifugal Elutriation Separation Chamber Radioactive Phosphorus Percoll Density Gradient Centrifugation 


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Copyright information

© Plenum Press, New York 1985

Authors and Affiliations

  • M. R. Hardeman
    • 1
  1. 1.Department of Internal Medicine Academic Medical CenterUniversity of AmsterdamThe Netherlands

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