Migratory Properties and Radiosensitivity of Lymphocytes

  • Jonathan Sprent
Part of the NATO ASI Series book series (NSSA, volume 88)


Radioactive isotopes have proven of enormous importance in studying nearly all aspects of lymphocyte physiology, including the origin, immune functions, migratory pathways, and life-spans of these cells. In this article I shall attempt to give a brief overview of lymphocyte physiology and function and then discuss the pros and cons of the various radioisotopes that have been used to study lymphocyte migration in vivo. Detailed reviews of lymphocyte physiology have appeared elsewhere (1–4).


Major Histocompatibility Complex Thoracic Duct Major Histocompatibility Complex Molecule White Pulp Exogenous Antigen 
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  1. 1.
    W. L. Ford, Lymphocyte migration and immune response, Prog Allergy 19: 1 (1975).PubMedGoogle Scholar
  2. 2.
    J. Sprent, Recirculating lymphocytes, in: “The Lymphocyte: Structure and Function”, ( J. J. Marchalonis, ed., Marcel Dekker, New York, (1977).Google Scholar
  3. 3.
    M. De Sousa, Lymphocyte Circulation: Experimental and Clinical Aspects, John Wiley & Sons, New York (1981).Google Scholar
  4. 4.
    D. M. V. Parrott and D. M. V. Wilkinson, Lymphocyte locomotion and migration, Prog Allergy 28: 193 (1981).PubMedGoogle Scholar
  5. 5.
    L. E. Hood, I. L. Weissman, W. B. Wood, “Immunology,” second ed., Benjamin/Cummings, CA 1984.Google Scholar
  6. 6.
    I. F. C. McKenzie and T. Potter, Murine lymphocyte surface antigens. Adv Immunol 27: 181 (1979).Google Scholar
  7. 7.
    E. Rheinherz and S. Schlossman, The differentiation and function of human lymphoctyes. Cell 19: 821 (1980).CrossRefGoogle Scholar
  8. 8.
    B. Pernis and H. J. Vogel, eds., “Regulatory T Lymphocytes”, Academic Press, New York (1980).Google Scholar
  9. 9.
    R. N. Zinkernagel and P. C. Doherty, MHC-restricted T cells: studies on the biological role of polymorphic major transplantation antigens determining T-cell restriction-specificity, function, and responsiveness, Adv Immunol 27: 51 (1979).PubMedCrossRefGoogle Scholar
  10. 10.
    D. C. Shreffler and C. S. David, The H-2 major histocompatibility complex and the I immune response region: genetic variation, function, and organization, Adv Immunol 20: 125 (1975).PubMedCrossRefGoogle Scholar
  11. 11.
    J. Klein and Z. A. Klein, MHC restriction and Ir genes, Adv Cancer Res 37: 233 (1982).PubMedCrossRefGoogle Scholar
  12. 12.
    J. Trowsdale, J. Lee, A. McMichael, HLA-DR bouillabaisse, Immunol Today 4: 31 (1983).CrossRefGoogle Scholar
  13. 13.
    N. Klinman, D. Mosier, I. Scher, E. Vitetta, eds., “B Lymphocytes in the Immune Response,” Elsevier (1981).Google Scholar
  14. 14.
    I. Sher, The CBA/N mouse strain: an experimental model illustrating the influence of the X-chromosome on immunity, Adv Immunol 33: 1 (1982).CrossRefGoogle Scholar
  15. 15.
    H. Cantor and I. L. Weissman, Development and function of thymocytes and T lymphocytes, Prog Allergy 20: 1 (1976).PubMedCrossRefGoogle Scholar
  16. 16.
    R. Scollary, Thymus cell migration: cells migrating from the thymus to peripheral lymphoid organs have a “mature” phenotype, J Immunol 128: 1566 (1982).Google Scholar
  17. 17.
    D. G. Osmond, The contribution of the bone marrow to the economy of the lymphoid system, Monogr Allergy 16: 57 (1980).Google Scholar
  18. 18.
    M. E. Smith and W. L. Ford, The recirculating lymphocyte pool of the rat: a systematic description of the migratory behaviour of recirculating lymphocytes, Immunology 49: 83 (1983).PubMedGoogle Scholar
  19. 19.
    W. M. Gallatin, I. L. Weissman, E. C. Butcher, A cell-surface molecule involved in organ-specific homing of lymphocytes, Nature 304: 30 (1983).PubMedCrossRefGoogle Scholar
  20. 20.
    D. Guy-Grand, C. Griscelli, P. Vassalli, The mouse gut T lymphocyte, a novel type of T cell. Nature, origin and traffic in mice in normal and graft-versus-host conditions, J Exp Med 148: 1661 (1978).PubMedCrossRefGoogle Scholar
  21. 21.
    R. E. Anderson and N. L. Warner, Ionizing irradiation and the immune response, Adv Immunol 24: 215 (1976).PubMedCrossRefGoogle Scholar
  22. 22.
    G. H. Rannie and K. J. Donald, Estimation of the migration of thoracic duct lymphocytes to nonlymphoid tissues: a comparison of the distribution of radioactivity at intervals following I.V. transfusion of cells labelled with H-3, Se-75, Tc-99m, I-125 and Cr-51 in the rat, Cell Tissue Kinet 10: 523 (1977).PubMedGoogle Scholar
  23. 23.
    W. L. Ford and M. E. Smith, in: “In Vivo Immunology,” A. A. van den Broek and M. G. Hanna, eds., Adv Exp Med Biol 149:139 (1982).Google Scholar
  24. 24.
    W. L. Ford, S. J. Simmonds, R. C. Atkins, Early events in systemic graft-versus-host reaction. II. Autoradio-graphic estimates of the frequency of donor lymphoctyes which respond to each Ag-B-determined antigenic complex, J Exp Med 141: 681 (1975).PubMedCrossRefGoogle Scholar
  25. 25.
    J. Sprent, Fate of H-2-activated T lymphocytes in syngeneic hosts. 1. Fate in lymphoid tissues and intestines traced with H-3-thymidine, I-125-deoxyuridine and Chromium-51, Cell Immunol 21: 278 (1976).PubMedCrossRefGoogle Scholar
  26. 26.
    J. G. Hall, An essay on lymphocyte circulation and the gut, Monogr Allergy 16: 100 (1980).PubMedGoogle Scholar
  27. 27.
    G. H. Rannie, M. L. Thakur, W. L. Ford, An experimental comparison of radioactive labels with potential application to lymphocyte migration studies in patients, Clin Exp Immunol 29: 509 (1977).PubMedGoogle Scholar
  28. 28.
    M. L. Rose and H. S. Micklem, SeL-75-selenomethionine: a new isotopic marker for lymphocyte localization studies, J Immunol Methods 9: 281 (1976).PubMedCrossRefGoogle Scholar
  29. 29.
    J. Wagstaff, C. Gibson, N. Thatcher, W. L. Ford, H. Sharma, D. Crowther, Human lymphocyte traffic assessed by Indium-111-oxine labeling: clinical observations, Clin exp Immunol 43: 443 (1981).PubMedGoogle Scholar
  30. 30.
    D. A. Goodwin, Cell labeling with oxine chelates of radioactive metal ions: techniques and clinical implications, J Nucl Med 19: 557 (1978).PubMedGoogle Scholar
  31. 31.
    T. Issekutz, W. Chin, J. B. Hay, Measurement of lymphocyte traffic with Indium-111, Clin exp Immunol 39: 215 (1980).PubMedGoogle Scholar
  32. 32.
    H. Frost, P. Frost, C. Wilcox, Lymph node scanning in sheep with Indium-111-labeled lymphocytes, Int J Nucl Med Biol 6: 60 (1979).PubMedCrossRefGoogle Scholar
  33. 33.
    J. P. Lavender, J. M. Goldman, R. B. Arnot, M. L. Thakur, Kinetics of Indium-111-labeled lymphocytes in normal subjects and patients with Hodgkin’s disease, Br Med J ii: 797 (1977).Google Scholar
  34. 34.
    S. M. Sparshot, H. Sharma, J. D. Kelley, W. L. Ford, Factors influencing the fate of Indium-111-labeled lymphocytes after transfer to syngeneic rats, J Immunol Methods 41: 303 (1981).CrossRefGoogle Scholar
  35. 35.
    R. J. M. ten Berge, A. T. Natarajan, M. R. Hardeman, E. A. van Royen, P. T. A. Schellekens, Labeling with Indium-111 has detrimental effect on human lymphocytes: concise communication, J Nucl Med 24: 615 (1983).PubMedGoogle Scholar
  36. 36.
    D. Forman and J. Rowly, Chromosomes and cancer, Nature 300: 403 (1982).PubMedCrossRefGoogle Scholar

Copyright information

© Plenum Press, New York 1985

Authors and Affiliations

  • Jonathan Sprent
    • 1
  1. 1.Department of ImmunologyScripps Clinic and Research CenterLa JollaUSA

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