Granulocyte Kinetics

  • A. M. Peters
  • S. H. Saverymuttut
  • J. P. Lavender
Part of the NATO ASI Series book series (NSSA, volume 88)


When DFP-32 (1—4) and later Cr-51 (5,6) were used to label granulocytes, much interest was generated in the field of granulocyte kinetics because the means became available to noninvasively quantify earlier direct observations made on living tissues. Such observations had revealed how granulocytes adhered to the endothelial surface of blood vessels, displaying a “rolling” motion along the surface (7,8).


Total Blood Volume Nylon Fiber Clearance Curve Bone Marrow Activity Splenic Blood Flow 
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  1. 1.
    J. W. Athens, A. M. Mauer, H. Ashenbrucker, G. E. Cartwright, M. M. Wintrobe, Leukokinetic studies. I. A method for labeling leukocytes with diisopropylfluorophosphate (DFP-32), Blood 14: 303 (1959).PubMedGoogle Scholar
  2. 2.
    A. M. Mauer, J. W. Athens, H. Ashenbrucker, G. E. Cartwright, M. M. Wintrobe, Leukokinetic studies. II. A method for labeling granulocytes in-vitro with radioactive diisopropylfluorophosphate, J Clin Invest 39: 1481 (1960).PubMedCrossRefGoogle Scholar
  3. 3.
    J. W. Athnes, A. M. Mauer, H. Ashenbrucker, G. E. Cartwright, M. M. Wintrobe. Leukokinetic studies. III. The distribution of granulocytes in the blood of normal subjects, J Clin Invest 40: 159 (1961).CrossRefGoogle Scholar
  4. 4.
    J. W. Athens, 0. P. Haab, S. 0. Raab, A. M. Mauer, H. Ashenbrucker, G. E. Cartwright, M. M. Wintrobe, Leukokinetic studies. IV. The total blood, circulating and marginal granulocyte pools and the granulocyte turnover rate in normal subjects, J Clin Invest 40: 989 (1961).PubMedCrossRefGoogle Scholar
  5. 5.
    R. McMillan and J. L. Scott, Leukocyte labeling with Chromium-51. I. Technique and results in normals, Blood 32: 738.Google Scholar
  6. 6.
    C. Drch, Y. Najean, J. Bauchet, Kinetic studies of Cr-51- and DF P-labeled granulocytes, Br J Haematol 29: 67 (1975).CrossRefGoogle Scholar
  7. 7.
    E. R. Clark, E. L. Clark, Observations on changes in blood vascular endothelium in the living animal, Am J Anat 57: 385 (1935).CrossRefGoogle Scholar
  8. 8.
    G. Vejlens, The distribution of leukocytes in the vascular system, Acta Pathol Microbiol Scand (Suppl 33 ) (1938).Google Scholar
  9. 9.
    J. Winkelman, C. J. Collica, S. G. Sandler, The delineation of abscesses by scintiphotography using Cr-51-labeled leukocytes. Am J Roentgenol Radium Ther Nucl Med 103: 881 (1968).PubMedGoogle Scholar
  10. 10.
    M. Deysine, R. G. Robinson, J. R. Wilder, Abscess detection by radioactive chromium-labeled autologous white cells, Surg Gynecol Obstet 131: 216 (1970).PubMedGoogle Scholar
  11. 11.
    W. C. Harvey and J. Silva, Detection of abscesses with Cr-51labeled leukocytes, J Nucl Med 15: 375 (1974).PubMedGoogle Scholar
  12. 12.
    R. L. Burleson, M. C. Johnson, H. Head, Scintigraphic demonstration of experimental abscesses with intravenous Ga-67 citrate and Ga-67-labeled blood leukocytes, Ann Surg 178: 446 (1973).PubMedCrossRefGoogle Scholar
  13. 13.
    R. L. Burleson, B. L. Holman, D. E. Tow, Scintigraphic demonstration of abscesses with radioactive gallium-labeled leukocytes, Surg Gynecol Obstet 141: 179 (1975).Google Scholar
  14. 14.
    T. Uchida, S. Kariyone, Organ distribution of the Tc-99m-labeled white cells, Acta Haematol Jap 36: 78 (1973).Google Scholar
  15. 15.
    D. English and B. R. Andersen, Organ distribution of canine leukocytes labeled with Tc-99m-sulfur colloid, J Nucl Med 18: 289 (1977).PubMedGoogle Scholar
  16. 16.
    H. J. Schroth, K. P. Muller, R. Berberich, E. Oberhausen, H. Wilhelm, The application of radioactive labeled leukocytes for the proof of inflammations, Eur J Nucl Med 4: 359 (1979).PubMedCrossRefGoogle Scholar
  17. 17.
    N. Colas-Linhart, M. Barbu, M. A. Gougerot, B. Bok, Five leukocyte labeling techniques: a comparative in vitro study, Br J Haematol 53: 31 (1983).PubMedCrossRefGoogle Scholar
  18. 18.
    J. G. McAfee and M. L. Thakur, Survey of radioactive agents for in vitro labeling of phagocytic leukocytes. I. Soluble agents, J Nucl Med 17: 480 (1976).PubMedGoogle Scholar
  19. 19.
    M. L. Thakur, J. P. Lavender, R. N. Arnot, D. J. Silvester, A. W. Segal, Indium-111-labeled autologous leukocytes in man. J Nucl Med 18: 1014 (1977).PubMedGoogle Scholar
  20. 20.
    M. L. Thakur, R. E. Coleman, M. J. Welch: Indium-111-labeled leukocytes for the localization of abscesses: preparation, analysis, tissue distribution and comparison with Gallium-67citrate in dogs, J Lab Clin Med 89: 217 (1977).PubMedGoogle Scholar
  21. 21.
    A. M. Peters, S. Karimjee, S.H. Saverymuttu, J. P. Lavender, A comparison of Indium-111-oxine and Indium-111-acetylacetonelabeled leukocytes in the diagnosis of inflammatory disease, Br J Radiol 55: 827 (1982).PubMedCrossRefGoogle Scholar
  22. 22.
    H. J. Danpure, S. Osman, F. Brady, The labeling of blood cells in plasma with In-ill-tropolonate, Br J Radiol 55: 247 (1982).PubMedCrossRefGoogle Scholar
  23. 23.
    S. H. Saverymuttu, A. M. Peters, H. J. Danpure, H. J. Reavy, S. Osman, J. P. Lavender, Lung transit of In-111-labeled granulocytes: relationship to labeling techniques, Scand J Haematol 30: 151 (1983).PubMedCrossRefGoogle Scholar
  24. 24.
    A. M. Peters, S. H. Saverymuttu, H. J. Reavy, H. J. Danpure, S. Osman, J. P. Lavender, Imaging inflammation with Indium-111tropolonate-labeled leukocytes, J Nucl Med 24: 39 (1983).PubMedGoogle Scholar
  25. 25.
    S. 0. Raab, J. W. Athens, 0. P. Haab, D. R. Boggs, H. Ashenbrucker, G. E. Cartwright, M. M. Wintrobe, Granulo-kinetics in normal dogs, Am J Physiol 206: 83 (1964).Google Scholar
  26. 26.
    P. C. Vincent, Granulocyte kinetics in health and disease, Clin Haematol 6: 695 (1977).PubMedGoogle Scholar
  27. 27.
    J. T. Dancey, K. A. Deubelbeiss, L. A. Harker, C. A. Finch, Neutrophil kinetics in man, J Clin Invest 58: 705 (1976).PubMedCrossRefGoogle Scholar
  28. 28.
    A. S. Deinard and A. R. Page, An improved method for performing neutrophil survival studies, Blood 36: 98 (1970).PubMedGoogle Scholar
  29. 29.
    P. C. Vincent, A. D. Chanana, E. P. Cronkite, D. D. Joel, The intravascular survival of neutrophils labeled in-vivo, Blood 43: 371 (1974).PubMedGoogle Scholar
  30. 30.
    B. J. Weiblen, L. Forstrom, J. McCullough, Studies of the kinetics of Indium-111-labeled granulocytes, J Lab Clin Med 94: 246 (1979).PubMedGoogle Scholar
  31. 31.
    J. G. McAfee, G. M. Gagne, G. Subramanian, Z. D. Grossman, F. D. Thomas, M. L. Roskopf, M. L. Fernandes, B. J. Lyons, Distribution of leukocytes labeled with In-lil-oxine in dogs with acute inflammatory lesions, J Nucl Med 21: 1059 (1980).PubMedGoogle Scholar
  32. 32.
    H. R. Bierman, K. H. Kelly, F. L. Cordes, The release of leukocytes and platelets from the pulmonary circulation by epinephrine, Blood 7: 683 (1952).PubMedGoogle Scholar
  33. 33.
    H. R. Bierman, R. L. Byron, K. H. Kelly, N. L. Petraki, The lung removal mechanism for leukocytes, Blood 6: 770 (1951).Google Scholar
  34. 34.
    B. A. Martin, J. L. Wright, H. Thomasen, J. C. Hogg, Effect of pulmonary blood flow on the exchange between the circulating and marginating pools of polymorphonuclear leukocytes in the dog lungs, J Clin Invest 69: 1277 (1982).PubMedCrossRefGoogle Scholar
  35. 35.
    A. S. Weisberger, R. W. Heinle, J. P. Storaasli, R. Hannah, Transfusion of leukocytes labeled with radioactive phosphorus, J Clin Invest 29: 336 (1950).PubMedCrossRefGoogle Scholar
  36. 36.
    S. H. Saverymuttu, A. M. Peters, H. J. Reavy, J. P. Lavender, Measurement of the migration and accumulation of granulocytes in inflammation in man, Clin Exp Immunol 52: 607 (1983).PubMedGoogle Scholar
  37. 37.
    A. C. Issekutz and H. Z. Movat, The in vivo quantitation and kinetics of rabbit neutrophil leukocyte accumulation in the skin in response to chemotactic agents and Escherichia Coli, Lab Invest 42: 310 (1980).PubMedGoogle Scholar
  38. 38.
    T. A. Lane, P. W. Bergum, J. P. Lichter, R. G. Spragg, The labeling of rabbit neutrophils with In-111-oxine, J Immunol Methods 51: 293 (1982).PubMedCrossRefGoogle Scholar
  39. 39.
    S. L. Poston, R. B. Jones, P. J. Hilton, Sodium transport in polymorphonuclear leukocytes: effect of isolation by the Ficoll/Triosil method, Clin Sci 62: 563 (1982).PubMedGoogle Scholar
  40. 40.
    R. Uchida and S. Kariyone, Intravascular granulocyte kinetics and spleen size in patients with neutropenia and chronic splenomegaly, J Lab Clin Med 82: 9 (1973).PubMedGoogle Scholar
  41. 41.
    C. R. Bishop, G. Rothstein, H. E. Ashenbrucker, J. W. Athens, Leukokinetic studies. XIV. Blood neutrophil kinetics in chronic steady state neutropenia, J Clin Invest 50: 1678.Google Scholar
  42. 42.
    L. H. Brubaker and C. A. Johnson, Correlation of splenomegaly and abnormal neutrophil pooling (margination), J Lab Clin Med 92: 508 (1978).PubMedGoogle Scholar
  43. 43.
    H. R. Bierman, R. L. Byron, K. H. Kelly, The role of the spleen in the leukocytosis following the intra-arterial administration of epinephrine, Blood 8: 153.Google Scholar
  44. 44.
    J. L. Spivak and S. Perry, Evaluation of Cr-S1 as a leukocyte label, Br J Haematol 25: 321 (1973).PubMedCrossRefGoogle Scholar
  45. 45.
    D. L. Williams, Improvement in quantitative data analysis by numerical deconvolution techniques, J Nucl Med 20: 568 (1979).PubMedGoogle Scholar
  46. 46.
    A. M. Peters and J. P. Lavender, Factors controlling the intrasplenic transit of platelets, Eur J Clin Invest 12: 191 (1982).PubMedCrossRefGoogle Scholar
  47. 47.
    S. H. Saverymuttu, A. M. Peters, J. P. Lavender, H. J. Hodgson, V. S. Chadwick, Indium-111-autologous leukocytes in inflammatory bowel disease, Gut 24: 293 (1983).PubMedCrossRefGoogle Scholar
  48. 48.
    S. H. Saverymuttu, A. M. Peters, H. J. Hodgson, V. S. Chadwick, J. P. Lavender, Indium-111 leukocyte scanning in small-bowel Crohn’s disease, Gastrointest Radiol 8: 157 (1983).PubMedCrossRefGoogle Scholar
  49. 49.
    P. Harris and D. Heath, The measurement of blood volume in the lungs, in: “The Human Pulmonary Circulation,” Churchill Livingstone, (1977).Google Scholar
  50. 50.
    A. Atherton and G. V. R. Born, Quantitative investigations of the adhesiveness of circulating polymorphonuclear leukocytes to blood vessels walls, J Physiol 222: 447 (1972).PubMedGoogle Scholar
  51. 51.
    A. Atherton and G. V. R. Born, Relationship between the velocity of rolling granulocytes and that of the blood flow in venules, J Physiol 233: 157 (1973).PubMedGoogle Scholar
  52. 52.
    P. Gorog, B. Kovacs, G. V. R. Born, Suppression of the intravascular adherence of granulocytes by N-acetyl neuraminic (sialic), acid Br J Exp Pathol 61: 490 (1980).Google Scholar
  53. 53.
    H. N. Mayrovitz, M. P. Weideman, R. F. Tuma, Factors influencing leukocyte adherence in microvessels. Thromb Haemost 38: 823 (1977).PubMedGoogle Scholar
  54. 54.
    L. Grant, The sticking and emigration of white blood cells in inflammation, in: “The Inflammatory Process,” B.W. Zweifach, L. Grant, R. T. McCluskey, eds., Academic Press, New York (1973).Google Scholar
  55. 55.
    R. R. MacGregor, P. J. Spagnuolo, A. L. Lentnek, Inhibition of granulocyte adherence by ethanol, prednisone and aspirin measured with an assay system, N Eng J Med 291: 642 (1974).CrossRefGoogle Scholar
  56. 56.
    J. E. Garvin, Factors affecting the adhesiveness of human leukocytes and platelets in vitro, J Exp Med 114: 51 (1961).PubMedCrossRefGoogle Scholar
  57. 57.
    K. J. Wozniak and E. M. Silverman, Granulocyte adherence in chronic alcoholism, Am J Clin Pathol 71: 269 (1979).PubMedGoogle Scholar
  58. 58.
    R. R. MacGregor, E. J. Macarak, N. A. Kefalides, Comparative adherence of granulocytes to endothelial monolayers, J Clin Invest 61: 697 (1978).PubMedCrossRefGoogle Scholar
  59. 59.
    J. D. Pearson, J. S. Carleton, J. E. Beesley, A. Hutchings, J. L. Gordon: Granulocyte adhesion to endothelium in culture, J Cell Sci 38: 225 (1979).PubMedGoogle Scholar
  60. 60.
    H. S. Jacob, P. R. Craddock, D. E. Hammerschmidt, C. F. Moldow, Complement-induced granulocyte aggregation: an unsuspected mechanism of disease, N Eng J Med 302: 789 (1980).CrossRefGoogle Scholar
  61. 61.
    J. C. Marsh, D. R. Boggs, G. E. Cartwright, M. M. Wintrobe, Neutrophil kinetics in acute infection, J Clin Invest 46: 1943 (1967).PubMedCrossRefGoogle Scholar
  62. 62.
    J. McCullough, B. J. Weiblen, M. E. Clay, L. Forstrom, Effect of leukocyte antibodies on the fate in vivo of Indium-111-labeled granulocytes, Blood 58: 164 (1981).PubMedGoogle Scholar
  63. 63.
    P. Murphy, “The Neutrophil,” Plenum Medical Book Co., London and New York (1976).Google Scholar

Copyright information

© Plenum Press, New York 1985

Authors and Affiliations

  • A. M. Peters
    • 3
  • S. H. Saverymuttut
    • 2
  • J. P. Lavender
    • 1
  1. 1.Departments of Diagnostic RadiologyLondonEngland
  2. 2.Medicine Hammersmith HospitalLondonEngland
  3. 3.Glaxo Group Research Ltd. WareHertfordshireEngland

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