Synthesis and Accumulation of Myosin Isozymes in Tissue Culture
Several questions can be asked concerning the synthesis and accumulation of myosin subunits and their isoforms in muscle cells grown in tissue culture. Do any of the transitions which characterize muscle development in vivo take place in tissue culture? Do myoblasts taken from animals of different ages, or from different muscle types synthesize the same myosin isozymes? Is morphological maturity of a muscle fiber in culture paralleled by accumulation of adult muscle myosins? We have examined each of these questions in three different tissue culture situations.
KeywordsSatellite Cell Organotypic Culture Tissue Culture System Myotube Formation Fast Myosin
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