Lipid Peroxidation Effects on Isolated Rat Retina
Often considered as a real peripheral expansion of the central nervous system, the retina, as a very differentiated sensory tissue, provides a biological material particularly accessible to experimentation. Moreover, it is new well established that the photoreceptor membranes are particularly rich in polyunsaturated fatty acids and therefore are especially sensitive to oxygenated free radicals (1). This is why, subsequent to Shvedova et al (2) experiments on the frog, we have studied the effects of lipid peroxidation on mammalian retinas. The work presented here was carried out on isolated albino rat retinas. Since Ames et al (3) and Weinstein et al (4)works, we knew that it is possible to maintain survival of an isolated rat retina supplying an electrophysiological response to light stimulation for several hours. The electro-retinogram (ERG) recorded is indeed the result of a succession of variations in membrane ionic permeability occurring at the level of the disc membrane and the plasma membrane of the rod outer segments (5). In these conditions, visual perception, associating a purely photochemical step and an electrophysiological step, presupposes absolute integrity of the photoreceptor membranes.
KeywordsLight Stimulation Photoreceptor Membrane Free Radical Scaven Absolute Integrity Sensory Tissue
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