On the Mechanism for Enhancement of Tumor Radiation to Hyperbaric Oxygen in Sodium Pentobarbital Anesthetized Rodents

  • John E. Biaglow
  • Herman D. Suit
  • Ralph E. Durand
  • Daniel E. Dosoretz
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 180)


Previously we have reported that the response of a C3H mouse mammary carcinoma to fractionated irradiation given while the mice respired pure oxygen at 3 atmospheres of pressure (3ATA) was markedly increased by combining with pentobarbital anesthesia (1). These results have been extended and confirmed employing a spontaneous fibrosarcoma (FSaII) and a spontaneous squamous cell carcinoma (SCCVII)(2). For those experiments radiation was given in 2 equal doses with 48–72 hours between fractions. Pentobarbital was administered in the amount of .05mg g-1 body weight intraperiotoneally several minutes before the animals were placed in the hyperbaric oxygen chamber. Oxygen (3ATA) was respired for 15 minutes prior to and then during the radiation treatment. In our examination for a mechanism for this anesthetic effect we have investigated the role of suppression of oxygen utilization at the dose levels of pentobarbital employed in those studies.


Oxygen Uptake Hyperbaric Oxygen Established Cell Line Radiation Response Oxygen Utilization 
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Copyright information

© Plenum Press, New York 1984

Authors and Affiliations

  • John E. Biaglow
    • 1
  • Herman D. Suit
    • 2
  • Ralph E. Durand
    • 3
  • Daniel E. Dosoretz
    • 4
  1. 1.Division of Radiation BiologyCase Western Reserve UniversityClevelandUSA
  2. 2.Edwin L. Steele Laboratory for Radiation Biology Department of Radiation Medicine Harvard Medical SchoolMassachusetts General HospitalBostonUSA
  3. 3.Section of RadiobiologyThe Johns Hopkins Oncology CenterBaltimoreUSA
  4. 4.Radiology and Radiation Therapy Regional CenterFort MyersUSA

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