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Increased Resolution Power in Po2 Analysis at Lower Po2 Levels Via Sensitivity Enhanced Optical Po2 Sensors (Po2 Optodes) using Fluorescence Dyes

  • N. Opitz
  • D. W. Lübbers
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 180)

Abstract

Po2 optodes (Lübbers and Opitz, 1975) are based on fluorescence quenching of suitable indicator molecules, e.g. pyrenebutyric acid (PBA) (Vaughan and Weber, 1970; Knopp and Longmuir, 1972), by molecular oxygen within a membrane or boundary layer protected indicator film of about 10/um thickness. Fig. 1 shows a couple of O2-depen-dent corrected excitation spectra of PBA (λ em = 395 nm) in a silisensitivity is drastically enhanced at lower PO2 values for the indicator membrane, whereas for PO2 values > 100 Torr the sensitivity of the indicator solution would be more favourable.

Keywords

Silicone Membrane Quenching Constant Indicator Solution Broad Band Excitation Indicator Molecule 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

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Copyright information

© Plenum Press, New York 1984

Authors and Affiliations

  • N. Opitz
    • 1
  • D. W. Lübbers
    • 1
  1. 1.Max-Planck-Institut für SystemphysiologieDortmundGermany

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