Abstract
An in vivo method of sister chromatid differentiation based on the intraperitoneal (ip) injection of bromodeoxyuridine (BrdUrd) previously adsorbed to activated charcoal is described, as well as the protocols for the use ot this method in spermatogonial,salivary gland and bone marrow cells, will be presented.
Results on sister chromatid exchange (SCE) induction obtained in bone marrow cells using well-known mutagens, especially cyclo phosphamide, are reported, and a comparison is made with the results obtained by other authors using different methods of BrdUrd administration.
The usefulness of this in vivo system for chemical mutagen detection and for chemical mutagen action on SCE induction is discussed.
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References
Allen, J.W., and S.A. Latt (1976) Analysis of sister chromatid exchange formation in vivo in mouse spermatogonia as a new test system for environmental mutagens. Nature 260: 449–451.
Allen, J.W., C.F. Schuler, R.W. Mendes, and S.A. Latt (1977) A simplified technique for in vivo analysis of sister chromatid exchanges using 5-bromodeoxyuridine tablets. Cytogen. Cell Genet. 18:231–237.
Barka, T., and H. Van der Noen (1975) Dissociation of rat paro tid gland. Lab. Invest. 32:373–375.
Kanda, N., and H. Kato (1979) A simple technique for in vivo observation of SCE in mouse ascites tumor and spermatogonial cells. Exp. Cell Res. 118:431–434.
Latt, A.S., J. Allen, S.E. Bloom, A. Carrano, E. Talke, D. Kram, E. Schneider, R. Schreck, R. Tice, B. Whitfield, S. Wolff (1981) Sister chromatid exchanges: A report of the gene-tox program. Mutat. Res. 87:17–62.
Morales-Ramírez, P. (1980) Analysis in vivo of sister chromatid exchange in mouse bone marrow and salivary gland cells. Mutat. Res. 74:61–69.
Nakanishi, Y., and E.L. Schenider (1979) In vivo sister-chromatid exchange: A sensitive measure of DNA damage. Mutat. Res .60:329–337.
Palitti, F., C. Tanzarella, R. Cozzi, R. Ricordy, E. Vitagliano, and M. Fiore (1982) Comparison of the frequencies of SCE induced by chemical mutagens in bone-marrow, spleen and spermatogonial cells of mice. Mutat. Res. 103:191–195.
Pera, F., and P. Mattias (1976) Labeling of DNA and differen tial sister chromatid staining after BrdU treatment in vivo. Chromosoma 57:13–18.
Perry, P., and S. Wolff (1974) New Giemsa method for the differential staining of sister chromatids. Nature 251:156–158.
Russer, G.C., and R.G. Tsanev (1973) Continuous labeling of mammalian DNA in vivo. Analyt. Biochem. 54:115–119.
Schneider, E.L., J.R. Chaillet, and R.R. Tice (1976) In vivo analysis of cellular replication. Proc. Natl. Acad. Sci., USA 74:2041–2044.
Tice R.R., Personal communication.
Vogel W., and T. Bauknecht (1976) Differential chromatid staining by in vivo treatment as a mutagenicity test system. Nature 260:448–449.
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© 1984 Plenum Press, New York
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Morales-Ramírez, P., Vallarino-Kelly, T., Rodríguez-Reyes, R. (1984). Detection of SCE in Rodent Cells Using the Activated Charcoal Bromodeoxyuridine System. In: Tice, R.R., Hollaender, A., Lambert, B., Morimoto, K., Wilson, C.M. (eds) Sister Chromatid Exchanges. Springer, Boston, MA. https://doi.org/10.1007/978-1-4684-4892-4_9
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DOI: https://doi.org/10.1007/978-1-4684-4892-4_9
Publisher Name: Springer, Boston, MA
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