Serum- and Plasma-Dependent Variations of Benzo(a)Pyrene-Induced Sister Chromatid Exchange in Human Lymphocytes
Sister chromatid exchange (SCE) is frequently used to assess the potential mutagenicity of chemical agents to human beings. We demonstrate here that levels of SCE induced by benzo(a)pyrene (BP) in the widely used blood lymphocyte assay are influenced by serum and plasma supplements. Sister chromatid exchange induction by BP was greatest when using fetal calf serum (FCS), intermediate with newborn calf serum (NCS), and lowest with autologous human plasma (AHP). This new finding adds to a growing list of factors capable of modulating the SCE response and underscores the need for re searchers to consider serum and plasma supplements in the standardization of the SCE approach in human mutagen assessments. The data also demonstrate the potential of SCE to aid in the study of serum factors which modify the mutagen sensitivity of human cells towards environmental carcinogens.
KeywordsFetal Calf Serum Human Lymphocyte Sister Chromatid Exchange Newborn Calf Serum Aryl Hydrocarbon Hydroxylase
Unable to display preview. Download preview PDF.
- 1.Carrano, A.V., and L.H. Thompson (1982) Sister chromatid exchange and single gene mutation. In Sister Chromatid Exchange, S. Wolff, ed. John Wiley and Sons, New York, pp. 59–86.Google Scholar
- 2.Abe, S., and M. Sasaki (1982) SCE as an index of mutagenesis and carcinogenesis. In Sister Chromatid Exchange, A.A. Sandberg, ed. Alan R. Liss, New York, pp. 461–514.Google Scholar
- 4.Carrano, A.V., and D.H. Moore (1982) The rationale and methodology for quantifying sister chromatid exchange in humans. In Mutagenicity: New Horizons in Genetic Toxicology, John Heddle, ed. Academic Press, New York, pp. 267–304.Google Scholar
- 5.Lindblad, A., K. Holmberg, and D. Francesconi (1982) The use of sister chromatid exchange to monitor human populations exposed to toxicologically harmful agents. In Sister Chromatid Exchange, S. Wolff, ed. John Wiley and Sons, New York, pp. 149–182.Google Scholar
- 11.Evans, H.J. (1982) Sister chromatid exchanges and disease states in man. In Sister Chromatid Exchange, S. Wolff, ed. John Wiley and Sons, New York, pp. 183–228.Google Scholar
- 15.Crossen, P.E. (1982) SCE in lymphocytes. In Sister Chromatid Exchange, A.A. Sandberg, ed. Alan R. Liss, New York, pp. 175–193.Google Scholar
- 24.Strazullo, B.P., A.M. Scanu, M.C. Ritter, A. Postiglone, and M. Mancini (1978) Binding, internalization, and degradation of human serum low density lipoproteins by human leukocytes in vitro: Effect on sterol metabolism. In International Confer ence on Atherosclerosis, L.A. Carson, R. Paoletti, C.R. Sitori, and G. Weber, eds. Raven Press, New York, pp. 477–483.Google Scholar
- 25.Beek, B., and G. Obe (1979) Sister chromatid exchange in human leukocyte chromosomes: Spontaneous and induced frequencies in early and late proliferating in vitro. Human Genet. 49:51–61.Google Scholar
- 27.Kellerman, G., E. Cantrell, and C.R. Shaw (1973) Variations in extent of arylhydrocarbon hydroxylase in cultured human lymphocytes. Cancer Res. 33:1654–1656.Google Scholar
- 29.Kouri, R.E., R.L. Inblum, R.G. Sosnowski, D.J. Slomiany, and C.E. McKinney (1979) Parameters influencing quantitation of 3-methylcholanthrene induced aryl-hydrocarbon hydroxylase activity in cultured human lymphocytes. J. Env. Path, and Toxicol. 2:1079–1098.Google Scholar
- 35.Lambert, B., A. Lindblad, K.G. Holmberg, and D. Francesconi (1982) The use of sister chromatid exchange to monitor human populations for exposure to toxicologically harmful agents. In Sister Chromatid Exchange, S. Wolff, ed. John Wiley and Sons, New York, pp. 149–151.Google Scholar