Importance of the Mitogen in Sister Chromatid Exchange Studies

  • Ghislain Deknudt


The occurrence of homologous exchanges between sister chroma tids (SCEs) was first demonstrated by Taylor (1) in autoradiographic studies on plant chromosomes labeled with tritiated thymidine. More recently, the pioneering work of Latt (2) on the substitution of thymidine by 5-bromo-2’-deoxyuridine (BrdUrd) and the application of the bis-benzimidazole fluorochrome Hoechst 33258, as well as the simultaneous development of the fluorescence-plus-Giesma (FPG) techniques by Kim (3), Korenberg and Freedlender (4), and Perry and Wolff (5), has provided differentially stained cytological preparations with a far greater resolution for SCE visualization than that obtained with autoradiography. These more sensitive techniques have led to an intensive proliferation of research in this new area of cytogenetics.


Human Lymphocyte Sister Chromatid Exchange Lens Culinaris Sister Chromatid Exchange Frequency Cell Cycle Duration 
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© Plenum Press, New York 1984

Authors and Affiliations

  • Ghislain Deknudt
    • 1
  1. 1.Laboratory of Mammalian Genetics Department of RadiobiologyS.C.K.-C.E.N.MolBelgium

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