Advertisement

Proliferative Kinetics and Chemical-Induced Sister Chromatid Exchanges in Human Lymphocyte Cultures

  • Kanehisa Morimoto

Abstract

Short-term cultures of phytohemagglutinin (PHA)-stimulated human lymphocytes are widely used to detect chromosome-damaging agents and possible human exposure to mutagenic carcinogens (1), and to study the immune response of blood (2,3). After stimulation of blood lymphocytes with PHA, the cultures soon contain different gen erations of cells, i.e., cells that have divided different numbers of times (4,5). This heterogeneity of cell division has been ascribed variously to a difference in cell cycle times, or to a difference in the times when the cells start blastogenesis in response to PHA (6–10).

Keywords

Sister Chromatid Human Lymphocyte Alkylating Agent Sister Chromatid Exchange Proliferative Kinetic 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Evans, H. J., and D.C. Lyoid (1978) Mutagen-Induced Chromosome Damage in Man. Yale University Press, New Haven.Google Scholar
  2. 2.
    Wedner, H.J., and C.W. Parker (1976) Lymphocyte activation. Prog. Allergy 20:195–300.PubMedCrossRefGoogle Scholar
  3. 3.
    Oppenheim, J.J., and D.L. Rosenstreich (1976) Signals regulat ing in vitro activation of lymphocytes. Prog. Allergy 20:65–194.PubMedCrossRefGoogle Scholar
  4. 4.
    Tice, R.R., E.L. Schneider, and E.L. Rary (1976) The utilization of bromodeoxyuridine incorporation into DNA for the analysis of cellular kinetics. Exp. Cell Res. 102:232–236.PubMedCrossRefGoogle Scholar
  5. 5.
    Morimoto, K., and S. Wolff (1980) Increase of sister chromatid exchanges and perturbations of cell division kinetics in human lymphocytes by benzene metabolites. Cancer Res. 40:1189–1193.PubMedGoogle Scholar
  6. 6.
    Tice, R., P. Thorne, and E.L. Schneider (1979) Bisack analysis of the phytohemagglutinin-induced proliferation of human peripheral lymphocytes. Cell Tissue Kinet. 12:1–9.PubMedGoogle Scholar
  7. 7.
    Crossen, P.E., and W.F. Morgan (1979) Proliferation of PHA-stimulated lymphocytes measured by combined antoradiography and sister chromatid differential staining. Exp. Cell Res. 118: 423–427.PubMedCrossRefGoogle Scholar
  8. 8.
    Craig-Holmes, A.P., and M.W. Shaw (1976) Cell cycle analysis in asynchronous cultures using the BUdR-Hoechst technique. Exp. Cell Res. 99:79–87.PubMedCrossRefGoogle Scholar
  9. 9.
    Soren, L. (1973) Variability of the time at which PHA-stimu-lated lymphocytes initiate DNA synthesis. Exp. Cell Res. 78: 201–208.PubMedCrossRefGoogle Scholar
  10. 10.
    Auf der Maur, P., and K. Berlincourt-Bohni (1979) Human lym phocyte cell cycle: Studies with the use of BrdUrd. Human Genet. 49:209–215.Google Scholar
  11. 11.
    Wolff, S., J. Bodycote, and R.B. Painter (1974) Sister chromatid exchanges induced in Chinese hamster cells by UV irradiation at different stages of the cell cycle: The necessity for cells to pass through S. Mutat. Res. 25:73–81.PubMedCrossRefGoogle Scholar
  12. 12.
    Morimoto, K. (1983) Induction of sister chromatid exchanges and cell division delays in human lymphocytes by microsomal activation of benzene. Cancer Res. 43:1330–1334.PubMedGoogle Scholar
  13. 13.
    Miura, K., K. Morimoto, and A. Koizumi (1983) Proliferative kinetics and mitomycin C-induced chromosome damage in Fanconi’s anemia lymphocytes. Human Genet. 63:19–23.CrossRefGoogle Scholar
  14. 14.
    Craig-Holmes, A.P., and M.W. Shaw (1977) Effects of six carcinogens on SCE frequency and cell kinetics in cultured human lymphocytes. Mutat. Res. 46:375–384.CrossRefGoogle Scholar
  15. 15.
    Samson, L., and J. Cairns (1977) A new pathway for DNA repair in Escherichia coli. Nature (Lond.) 267:281–283.CrossRefGoogle Scholar
  16. 16.
    Robins, P., and J. Cairns (1979) Quantitation of the adaptive response to alkylating agents. Nature (Lond.) 280:74–76.CrossRefGoogle Scholar
  17. 17.
    Karran, P., and T. Lindahl (1979) Adaptive response to alkylating agents involves alteration in situ of 06-methylguanine residues in DNA. Nature (Lond.) 280:76–77.CrossRefGoogle Scholar
  18. 18.
    Samson, L., and J.L. Schwartz (1980) Evidence for an adaptive DNA repair pathway in Chinese hamster ovary and human skin fibroblast cell lines. Nature (Lond.) 287:861–863.CrossRefGoogle Scholar
  19. 19.
    Montesano, R., H. Bresil, and G.P. Margison (1979) Increased excision of O6-methylguanine from rat liver DNA after chronic administration of dimethylnitrosoamine. Cancer Res. 39:1798–1802.PubMedGoogle Scholar
  20. 20.
    Montesano, R., H. Bresil, G.P. Plnch-Martel, and A.E. Pegg (1980) Effects of chronic treatment of rats with dimethylnitrosoamine on the removal of 06-methylguanine from DNA. Cancer Res. 40:452–458.PubMedGoogle Scholar
  21. 21.
    Bridges, B., and A. Lehmann (1982) Inducible responses to DNA damage. Nature (Lond.) 298:118.CrossRefGoogle Scholar
  22. 22.
    Evensen, G., and E. Seeberg (1982) Adaptation of alkylation resistance involves the induction of a DNA glycosylase. Nature (Lond.) 296:773:775.CrossRefGoogle Scholar
  23. 23.
    Waldstein, E.A., E-H. Cao, and R.B. Setlow (1982) Adaptive increase of 06-methylguanine-acceptor protein in HeLa cells following N-methyl-N’-nitro-N-nitrosoguanidine treatment. Nucleic Acids Res. 10:4595–4604.PubMedCrossRefGoogle Scholar
  24. 24.
    Sirover, M.A. (1979) Induction of the DNA repair enzyme urasil-DNA glycosylase in stimulated human lymphocytes. Cancer Res. 39:2090–2095.PubMedGoogle Scholar
  25. 25.
    Waldstein, E.A., E-H. Cao, M.A. Bender, and R.B. Setlow (1982) Abilities of extracts of human lymphocytes to remove 06-methyl-guanine from DNA. Mutat. Res. 95:405–416.PubMedCrossRefGoogle Scholar
  26. 26.
    Goto, K., Y. Maeda, Y. Kano, and T. Sugiyama (1978) Factors involved in differential Giemsa-staining of sister chromatids. Chromosoma 66:351–359.PubMedCrossRefGoogle Scholar
  27. 27.
    Morimoto, K., and S. Wolff (1980) Cell cycle kinetics in human lymphocyte cultures. Nature (Lond.) 288:604–606.CrossRefGoogle Scholar
  28. 28.
    Rutledge, M.H. (1979) A simple procedure for obtaining auto-radiographs of G-banded chromosomes. Chromosoma 70: 259–262.PubMedCrossRefGoogle Scholar
  29. 29.
    Morimoto, K., M. Sato, and A. Koizumi (1983) Proliferative kinetics of human lymphocytes in culture measured by autoradiography and sister chromatid differential staining. Exp. Cell. Res. 145:349–356.PubMedCrossRefGoogle Scholar
  30. 30.
    Sasaki, M.S. (1978) Radiation damage and its repair in the formation of chromosome aberrations in human lymphocytes. In Mutagen-Induced Chromosome Damage in Man, H.J. Evans and D.C. Lloyd, eds. Yale University Press, New Haven, pp. 62–76.Google Scholar
  31. 31.
    Wolff, S. (1972) The repair of X-ray-induced chromosome aberrations in stimulated and unstimulated human lymphocytes. Mutat. Res. 15:435–444.PubMedCrossRefGoogle Scholar
  32. 32.
    Wolff, S. (1978) Chromosomal effects of mutagenic carcinogens and the nature of lesions leading to sister chromatid exchange. In Mutagen-Induced Chromosome Damage in Man, H.J. Evans and D.C. Lloyd, eds., Yale University Press, New Haven, pp. 208–215.Google Scholar
  33. 33.
    Natarajan, A.T., A.D. Tates, M. Meijers, I. Neuteboom, and N. de Vogel (1983) Induction of sister chromatid exchanges (SCEs) and chromosomal aberrations by mitomycin C and methyl methane-sulfonate in Chinese hamster ovary cells. An evaluation of methodology for detection of SCEs and of persistent DNA lesions towards the frequencies of observed SCEs. Mutat. Res. 121:211–223.PubMedCrossRefGoogle Scholar
  34. 34.
    Natarajan, A.T., and G. Obe (1982) Mutagenicity testing with cultured mammalian cells: Cytogenic assays. In Mutagenicity -New Horizons in Genetic Toxicology, J.A. Heddle, ed. Academic Press, New York, pp. 551–559.Google Scholar
  35. 35.
    Littlefield, L.G., S.P. Colayer, and R.J. DuFrain (1983) SCE evaluations in human lymphocytes after GO exposure to mitomycin C: Lack of expression MMC induced SCEs in cells that have undergone greater than two in vitro divisions. Mutat. Res. 107:119–130.PubMedCrossRefGoogle Scholar
  36. 36.
    Samson, L., and J.L. Schwartz (1983) The induction of resistance to alkylation damage in mammalian cells. (submitted for publication)Google Scholar

Copyright information

© Plenum Press, New York 1984

Authors and Affiliations

  • Kanehisa Morimoto
    • 1
  1. 1.Department of Public Health Faculty of MedicineUniversity of TokyoTokyo 113Japan

Personalised recommendations