Proliferative Kinetics and Chemical-Induced Sister Chromatid Exchanges in Human Lymphocyte Cultures
Short-term cultures of phytohemagglutinin (PHA)-stimulated human lymphocytes are widely used to detect chromosome-damaging agents and possible human exposure to mutagenic carcinogens (1), and to study the immune response of blood (2,3). After stimulation of blood lymphocytes with PHA, the cultures soon contain different gen erations of cells, i.e., cells that have divided different numbers of times (4,5). This heterogeneity of cell division has been ascribed variously to a difference in cell cycle times, or to a difference in the times when the cells start blastogenesis in response to PHA (6–10).
KeywordsSister Chromatid Human Lymphocyte Alkylating Agent Sister Chromatid Exchange Proliferative Kinetic
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