Measurement and Inhibition of Membrane Transport of Adenosine

  • Alan R. P. Paterson
  • Eric R. Harley
  • Carol E. Cass


The effects of adenosine on a variety of physiological processes in cells and tissues have been widely interpreted in terms of regulatory roles for adenosine. These effects are mediated by the presence of adenosine (or certain related compounds) on extracellular adenosine receptors on the responsive cells. An understanding of such apparent regulatory actions of adenosine will require not only knowledge of the biochemical events linking receptor occupancy and the cellular response, but also an understanding of the source and delivery of adenosine molecules to receptors and of their clearance from the immediate vicinity of the receptors. Cellular utilization appears to be a principal means of clearing of extracellular adenosine from the vicinity of receptors. Because adenosine and other physiological nucleosides leave and enter cells mainly by way of nucleoside-specific transport mechanisms, transport is a primary step both in the formation and in the disposition of extracellular adenosine. This chapter is concerned with the measurement of adenosine transport in cell suspensions and with the potent inhibition of this process by several agents. Because of the emphasis of this volume on methodology, the material presented is selective rather than comprehensive.


Human Erythrocyte Cellular Accumulation Nucleoside Transport Mouse Lymphoma Cell L5178Y Cell 
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Copyright information

© Plenum Press, New York 1985

Authors and Affiliations

  • Alan R. P. Paterson
    • 1
  • Eric R. Harley
    • 1
  • Carol E. Cass
    • 1
  1. 1.Cancer Research Group McEachern LaboratoryUniversity of AlbertaEdmontonCanada

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