The Study of Adenosine Metabolism in Isolated Cells and Tissues
Meaningful studies of adenosine metabolism in isolated animal cells and tissues rely on a thorough understanding of the pathways of adenosine metabolism, on an appreciation of the complications involved in measuring the rates of these pathways, and on a knowledge of the criteria that must be met by methods for the preparation of biological systems and for incubation and extraction. These points are considered herein, together with the several experimental approaches to the study of adenosine metabolism that are commonly used; in addition, a number of methods for the separation of adenosine and its metabolites are given.
KeywordsAdenine Nucleotide Adenosine Deaminase Purine Metabolism Ehrlich Ascites Tumor Cell Adenosine Kinase
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