Use of Photoaffinity Labels as P2-Purinoceptor Antagonists

  • Jeffrey S. Fedan
  • G. Kurt Hogaboom
  • John P. O’Donnell
  • David P. Westfall


In recent years there has been considerable interest in the possible role of adenine nucleotides, such as ATP, as neuromodulators (Su, 1977; De Mey et al., 1979; Katsuragi and Su, 1982), neurotransmitters (Burnstock et al., 1970; Burnstock, 1979), or cotransmitters (Westfall et al., 1978; Fedan et al, 1981; Sneddon et al., 1982a). The chief impediment to accepting the notion that adenine nucleotides act as neuromodulators or neurotransmitters has been the unavailability of a specific pharmacological antagonist of responses to ATP (see, for example, Campbell and Gibbons, 1979). Although a number of compounds have been investigated in this regard, including 2-2′-pyridylisatogen, 2-2′-methoxyphenylisatogen, quinidine, apamin, and 2-substituted imidazolines, the antagonism afforded by these drugs in several autonomic nerve-smooth muscle preparations is nonspecific; i.e., in concentrations sufficient to antagonize responses to ATP, the responses to other agonists are also reduced (Weetman and Turner, 1977; Burnstock, 1979, 1983).


Adenine Nucleotide Photoaffinity Label Smooth Muscle Preparation Organ Chamber Filter Flask 
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Copyright information

© Plenum Press, New York 1985

Authors and Affiliations

  • Jeffrey S. Fedan
    • 1
  • G. Kurt Hogaboom
    • 2
  • John P. O’Donnell
    • 3
  • David P. Westfall
    • 4
  1. 1.Physiology SectionNational Institute for Occupational Safety and HealthUSA
  2. 2.Department of Pharmacology and ToxicologyWest Virginia University Medical CenterMorgantownUSA
  3. 3.School of PharmacyWest Virginia University Medical CenterMorgantownUSA
  4. 4.Department of PharmacologyUniversity of Nevada School of MedicineRenoUSA

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