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Organization of Glycoproteins within the Platelet Plasma Membrane

  • Thomas J. Kunicki

Abstract

Many processes within the eukaryotic cell membrane require the formation of functional macromolecular complexes from individual membrane or cytoplasmic proteins. In light of accumulating evidence that specific membrane glycoproteins function as receptors in critical stages of the platelet adhesion and aggregation mechanisms, there is currently much interest in the organization of proteins within the human platelet membrane and the changes that occur in this organization during platelet function. Within the last ten years, analytical methods such as sodium dodecyl sulfate-poly-acrylamide gel electrophoresis (SDS-PAGE) in one or two dimensions (Phillips and Poh Agin, 1977a) and combined isoelectric focusing-SDS/PAGE (Clemetson et al., 1979) have proved to be extremely valuable for the resolution, identification, and characterization of membrane glycoproteins (see Chapter 3). Thus, Phillips and Poh Agin (1977a) were able to establish molecular criteria for the identification of seven “major” membrane glycoproteins based on their differential mobilities in the presence or absence of disulfide bond reduction. When such analytical methods were used to compare platelet proteins from normal individuals and patients with inherited abnormalities of platelet function, the link between certain membrane glycoproteins and selected platelet functions was established. For example, Glanzmann’s thrombasthenia, which is characterized by defective platelet cohesion, is associated with the absence of or a qualitative defect in glycoproteins (GP) lib and Ilia (Nurden and Caen, 1974; Phillips and Poh Agin, 1977b; Lightsey et al, 1981).

Keywords

Human Platelet Platelet Membrane Platelet Lysate Fibrinogen Binding Platelet Protein 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Plenum Press, New York 1985

Authors and Affiliations

  • Thomas J. Kunicki
    • 1
  1. 1.The Blood Center of Southeastern Wisconsin, IncMilwaukeeUSA

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