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The Insulin Receptor Protein Kinase

  • C. Ronald Kahn
  • Morris F. White
  • Florin Grigorescu
  • Sumiko Takayama
  • Hans U. Häring
  • Marco Crettaz

Abstract

Phosphorylation reactions catalyzed by protein kinases rapidly and reversibly modify the activity of cellular enzymes. Considerable evidence indicates that phosphorylation and dephosphorylation of proteins play an important role in the action of insulin and other hormones.(1–4) Receptors for several growth factors and neurotransmitters(5–14) are also phosphorylated, and in some cases this phosphorylation is increased after binding of the hormone. In addition, protein kinases are associated with certain transforming retroviruses that have profound effects on cellular growth and metabolism.(14–20) These findings suggest that phosphorylation may be an early step in the transmembrane signal process. In 1981, we began to study the possibility that the insulin receptor undergoes a phosphorylation reaction.(21) In this chapter, we review briefly the structure of the insulin receptor kinase, describe the kinetic characteristics of the receptor kinase measured in vitro, compare and contrast the phosphorylation of the receptor in broken and intact cells, and finally describe the phosphorylation of the receptor in certain insulin-resistant states.

Keywords

Insulin Receptor Hepatoma Cell Intact Cell Insulin Binding Receptor Phosphorylation 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Plenum Press, New York 1985

Authors and Affiliations

  • C. Ronald Kahn
    • 1
  • Morris F. White
    • 1
  • Florin Grigorescu
    • 1
  • Sumiko Takayama
    • 1
  • Hans U. Häring
    • 1
  • Marco Crettaz
    • 1
  1. 1.Research Division, Joslin Diabetes Center and Department of MedicineBrigham and Women’s Hospital, Harvard Medical SchoolBostonUSA

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