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Insulin-Dependent Apparent Translocation of Glucose Transport Activity: Studies by the Reconstitution Method

  • Tetsuro Kono

Abstract

Nearly a decade ago, we found that when rat epididymal adipocytes were exposed to [125I]iodoinsulin for 10 min at 37°C, 125I activity would associate with two subcellular structures that could be fractionated by sucrose density-gradient centrifugation into the plasma membrane fraction (peak 1) and a low-density microsomal fraction (peak 2).(1) The latter peak (peak 2 of 125I activity) was later identified as the peak of the internalized hormone.(2,3) During this study, however, we noticed several similarities between the characteristics of the peak 2 formation, i,e., the endocytotic internalization of the hormone-receptor complex, and those of the hormonal action on glucose transport. Thus, both reactions are very slow at a low temperature, such as 15°C,(2) both reactions require adenosine triphosphate (ATP) not only for their development(2,3) but also for their reversal,(4,5) and both reactions are completed in approximately 5–10 min at 37°C when the hormone concentration is 1 nM.(2,6) We therefore postulated as a working hypothesis that the complex of glucose and its transport carrier might be co-internalized along with the insulin-receptor complex. In theory, one can test this working hypothesis by measuring the distribution of glucose transport activity in subcellular fractions obtained from the basal and plus-insulin states of adipocytes using the aforementioned sucrose density-gradient centrifugation.

Keywords

Subcellular Fraction Insulinlike Effect Glucose Transport Activity States Public Health Service Grant Basal Glucose Transport 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Plenum Press, New York 1985

Authors and Affiliations

  • Tetsuro Kono
    • 1
  1. 1.Department of Physiology, School of MedicineVanderbilt UniversityNashvilleUSA

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