Hexose Transport Regulation by Insulin in the Isolated Rat Adipose Cell

  • Ian A. Simpson
  • Samuel W. Cushman

Abstract

Following the initial observations that insulin stimulates the metabolism of glucose in a variety of peripheral tissues,(1) it was soon recognized that this stimulatory effect was exerted at the level of glucose uptake.(2–5) Since these earlier observations, most studies pertaining to the action of insulin on glucose uptake have been carried out in adipose tissue and, more specifically, rat adipose tissue. Despite its relatively small contribution to glucose homeostasis in the whole animal,(6) adipose tissue has been preferred for such studies because of the ease with which it can be obtained, the ability to prepare isolated adipose cells relatively free of other cell types,(7) and the extreme sensitivity and responsiveness of the adipose cell to insulin. Validation of the adipose cell as a model for studies of the mechanism of insulin action on hexose transport is now being obtained by parallel studies in muscle.(8,9) However, significant and even marked differences in the magnitude and sensitivity of the response to insulin are apparent between tissues, as they are between adipose cells of different species such as guinea pig(10) and man.(11)

Keywords

Adenosine Fractionation Catecholamine Glucagon Photolysis 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Wertheimer, E., and Shapiro, B., 1948, Physiol. Rev. 28: 451–464.PubMedGoogle Scholar
  2. 2.
    Winegrad, A.I., and Renold, A.E., 1958, J. Biol. Chem. 233: 267–272.Google Scholar
  3. 3.
    Vaughan, M., 1961, J. Lipid Res. 2: 293–316.Google Scholar
  4. 4.
    Crofford, O.B., and Renold, A.E., 1965, J. Biol. Chem. 240: 14–21.PubMedGoogle Scholar
  5. 5.
    Crofford, O.B., and Renold, A.E., 1965, J. Biol. Chem. 240: 3237–3244.PubMedGoogle Scholar
  6. 6.
    Rabinowitz, D., 1970, Annu. Rev. Med. 21: 241–258.PubMedCrossRefGoogle Scholar
  7. 7.
    Rodbell, M., 1964, J. Biol. Chem. 239: 375–380.PubMedGoogle Scholar
  8. 8.
    Wardzala, L.J., and Jeanrenaud, B., 1981, J. Biol. Chem. 256: 7090–7093.PubMedGoogle Scholar
  9. 9.
    Wardzala, L.J., and Jeanrenaud, B., 1983, Biochim. Biophys. Acta 730: 49–56.PubMedCrossRefGoogle Scholar
  10. 10.
    Horuk, R., Rodbell, M., Cushman, S.W., and Wardzala, L.J., 1983, J. Biol. Chem. 258: 7425–7429.PubMedGoogle Scholar
  11. 11.
    Cushman, S.W., Kamieli, E., Foley, J.E., Hissin, P.J., Simpson, I.A., and Salans, L.B., 1982, Clin. Res. 30: 388A.Google Scholar
  12. 12.
    Vinten, J., Gliemann, J., and Osterlind, K., 1976, J. Biol. Chem. 251: 794–800.PubMedGoogle Scholar
  13. 13.
    Wardzala, L.J., Cushman, S.W., and Salans, L.B., 1978, J. Biol. Chem. 253: 8002–8005.PubMedGoogle Scholar
  14. 14.
    Cushman, S.W., and Wardzala, L.J., 1980, J. Biol. Chem. 255: 4758–4762.PubMedGoogle Scholar
  15. 15.
    Kamieli, E., Zamowski, M.J., Hissin, P.J., Simpson, I.A., Salans, L.B., and Cushman, S.W., 1981, J. Biol. Chem. 256: 4772–4777.Google Scholar
  16. 16.
    Suzuki,K.,and Kono,T.,1980,Proc. Natl. Acad. Sci. USA77: 2542–2545.PubMedCrossRefGoogle Scholar
  17. 17.
    Kono T., Suzuki, L., Dansey, L.E., Robinson, F.W., and Blevins, T.L., 1981, J. Biol. Chem. 256: 6400–6407.PubMedGoogle Scholar
  18. 18.
    Kono, T., Robinson, F.W., Blevins, T.L., and Ezaki, 0., 1982, J. Biol. Chem. 257: 10942–10947.PubMedGoogle Scholar
  19. 19.
    Foley, J.E., Cushman, S.W., and Salans, L.B., 1978, Am. J. Physiol. 234: E112 — E119.PubMedGoogle Scholar
  20. 20.
    Livingston, J.N., and Lockwood, D.H., 1974, Biochem. Biophys. Res. Commun. 61: 989–996.PubMedCrossRefGoogle Scholar
  21. 21.
    Olefsky, J.M., 1975, J. Clin. Invest. 56: 1499–1508.PubMedCrossRefGoogle Scholar
  22. 22.
    Loten, E.G., Regen, D.M., and Park, C.R., 1976, J. Cell Physiol. 89: 651–659.PubMedCrossRefGoogle Scholar
  23. 23.
    Gliemann J., Osterlind, K., Vinten, J., and Gammeltoft, S., 1972, Biochim. Biophys. Acta 286: 1–9.PubMedCrossRefGoogle Scholar
  24. 24.
    Gliemann, J., and Rees, W.D., 1983, Curr. Top. Membr. Transp. 18: 339–379.CrossRefGoogle Scholar
  25. 25.
    Häring, J.V., Kemmler, W., Renner, R., and Hepp, K.D., 1978, FEBS Lett. 95: 177–180.PubMedCrossRefGoogle Scholar
  26. 26.
    Ciaraldi, T.P., and Olefsky, J.M., 1979, Arch. Biochem. Biophys. 193: 221–231.PubMedCrossRefGoogle Scholar
  27. 27.
    Kono, T., and Barham, F.W., 1971, J. Biol. Chem. 246: 6210–6216.PubMedGoogle Scholar
  28. 28.
    McKeel, D.W., and Jarett, L., 1970, J. Cell Biol. 44: 417–432.PubMedCrossRefGoogle Scholar
  29. 29.
    Simpson, I.A., Yver, D.R., Hissin, P.J., Wardzala, L.J., Kamieli, E., Salans, L.B., and Cushman, S.W., 1984, Biochim. Biophys. Acta763: 393–407.CrossRefGoogle Scholar
  30. 30.
    Kasahara, M., and Hinkle, P.C., 1976, Proc. Natl. Acad. Sci. USA 73: 396–400.PubMedCrossRefGoogle Scholar
  31. 31.
    Wardzala, L.J., 1979, Identification of the Glucose Transport System in Purified Rat Adipose Cell Plasma Membranes Using a Cytochalasin B Binding Assay: Effects of Insulin and Altered Physiological States,Ph.D. dissertation, Dartmouth College, Hanover, New Hampshire.Google Scholar
  32. 32.
    Cushman, S.W., Wardzala, L.J., Simpson, I.A., Kamieli, E., Hissin, P.J., Wheeler, T.J., Hinkle, P.C., and Salans, L.B., 1984, Fed. Proc. 43: 2251–2255.PubMedGoogle Scholar
  33. 33.
    Simpson, I.A., Zamowski, M.J., and Cushman, S.W., 1983, Fed. Proc. 42: 1790 (abstr.).Google Scholar
  34. 34.
    Simpson, I.A., Karnieli, E., Hissin, P.J., Smith, U., and Cushman, S.W., 1984, Proc. Gen. Physiol. Soc. USA38 (in press).Google Scholar
  35. 35.
    Simpson, I.A., Martin, M.L., and Cushman, S.W., 1982, Diabetes 31 (Suppl. 2): 2A.Google Scholar
  36. 36.
    Wardzala, L.J., Simpson, I.A., Rechler, M.M., and Cushman, S.W., 1984, J. Biol. Chem. 259: 8378–8383.Google Scholar
  37. 37.
    Wang, C.-C, Sonne, O., Hedo, J.A., Cushman, S.W., and Simpson, I.A., 1983, J. Biol. Chem. 258: 5129–5134.Google Scholar
  38. 38.
    Sonne, O., and Simpson, I.A., 1984, Biochem. Biophys. Acta804: 404–413.Google Scholar
  39. 39.
    Simpson, I.A., Hedo, J.A., and Cushman, S.W., 1984, Diabetes 33:13–18. 1st.).Google Scholar
  40. 40.
    Hedo, J.A., and Simpson, I.A., 1984, J. Biol. Chem. 259: 11083–11089.Google Scholar
  41. 41.
    Taylor, W.M., Mak, M., and Halperin, M.L., 1976, Proc. Natl. Acad. Sci. USA 73: 4359–4363.Google Scholar
  42. 42.
    Kashiwagi, A., and Foley, J.E., 1982, Biochem. Biophys. Res. Commun. 107: 1151–1157.Google Scholar
  43. 43.
    Kashiwagi, A., Heucksteadt, T.P., and Foley, J.E., 1983, J. Biol. Chem. 258: 13685–13692.Google Scholar
  44. 44.
    Kirsch, D.M., Baumgarten, M., Deufel, I., Rinninger, F., Kemmler, W., and Hazing, H.U., 1984, Biochem. J. 217: 737–745.Google Scholar
  45. 45.
    Green, A., 1983, Biochem. J. 212: 189–195.PubMedGoogle Scholar
  46. 46.
    Smith, U., Kuroda, M., and Simpson, I.A., 1984, J. Biol. Chem. 259: 8758–8763.PubMedGoogle Scholar
  47. 47.
    Ludvigsen, C., Jarett, L., and McDonald, J.M., 1980, Endocrinology 106: 786–790.PubMedCrossRefGoogle Scholar
  48. 48.
    Londos, C., Cooper, D.M.F., Schlegel, W., and Rodbell, M., 1978, Proc. Natl. Acad. Sci. USA 75: 5362–5366.PubMedCrossRefGoogle Scholar
  49. 49.
    Pessin, J.E., Gitomer, W., Oka, Y., Oppenheimer, C.L., and Czech, M.P., 1983, J. Biol. Chem. 258: 7386–7394.PubMedGoogle Scholar
  50. 50.
    Lönnroth, P., and Smith, U., 1983, Biochem. Biophys. Res. Commun. 112: 971–979.CrossRefGoogle Scholar
  51. 51.
    Kirsch, D.M., Kemler, W., and Häring, M.U., 1983, Biochem. Biophys. Res. Commun. 115: 398–405.PubMedCrossRefGoogle Scholar
  52. 52.
    Karnieli, E., Hissin, P.J., Simpson, I.A., Salans, L.B., and Cushman, S.W., 1981, J. Clin. Invest. 68: 811–814.PubMedCrossRefGoogle Scholar
  53. 53.
    Hissin, P.J., Foley, J.E., Wardzala, L.J., Karnieli, E., Simpson, I.A., Salans, L.B., and Cushman, S.W., 1982, J. Clin. Invest. 70: 780–790.PubMedCrossRefGoogle Scholar
  54. 54.
    Salans, L.B., Foley, J.E., Wardzala, L.J., and Cushman, S.W., 1981, Am. J. Physiol. 240: E175 - E183.PubMedGoogle Scholar
  55. 55.
    Hissin, P.J., Karnieli, E., Simpson, I.A., Salans, L.B., and Cushman, S.W., 1982, Diabetes 31: 589–592.PubMedCrossRefGoogle Scholar
  56. 56.
    Kahn, B.B., and Cushman, S.W., 1984, Diabetes 33 (Suppl. 1): 71A.Google Scholar
  57. 57.
    Kahn, B.B., Horton, E.S., and Cushman, S.W., 1984, Clin. Res. 32: 399A.Google Scholar
  58. 58.
    Kasahara, M., and Hinkle, P.C., 1977, J. Biol. Chem. 252: 7384–7390.PubMedGoogle Scholar
  59. 59.
    Gorga, F.R., Baldwin, S.A., and Lienhard, G.E., 1979, Biochem. Biophys. Res. Commun. 91: 955–961.PubMedCrossRefGoogle Scholar
  60. 60.
    Sogin, D.C., and Hinkle, P.C., 1980, Proc. Natl. Acad. Sci. USA 77: 5725–5729.PubMedCrossRefGoogle Scholar
  61. 61.
    Baldwin, S.A., and Lienhard, G.E., 1981, Trends Biochem. Sci. 6: 208–211.CrossRefGoogle Scholar
  62. 62.
    Wheeler, T.J., Simpson, I.A., Sogin, D.C., Hinkle, P.C., and Cushman, S.W., 1982, Biochem. Biophys. Res. Commun. 105: 89–95.PubMedCrossRefGoogle Scholar
  63. 63.
    Lienhard, G.E., Kin, H.K., Ransome, K.J., and Gorga, J.C., 1982, Biochem. Biophys. Res. Commun. 105: 1150–1156.PubMedCrossRefGoogle Scholar
  64. 64.
    Carter-Su, C., Pessin, J.E., Mora, E., Gitomer, W., and Czech, M.P., 1982, J. Biol. Chem. 257: 5419–5425.PubMedGoogle Scholar
  65. 65.
    Shanahan, M.F., Olson, S.A., Weber, M.J., Lienhard, G.E., and Gorga, J.C., 1982, Biochem. Biophys. Res. Commun. 107: 38–43.PubMedCrossRefGoogle Scholar
  66. 66.
    Horuk, R., Rodbell, M., Cushman, S.W., and Simpson, I.A., 1983, FEBS Lett.164:261–266PubMedCrossRefGoogle Scholar

Copyright information

© Plenum Press, New York 1985

Authors and Affiliations

  • Ian A. Simpson
    • 1
  • Samuel W. Cushman
    • 1
  1. 1.Cellular Metabolism and Obesity Section, National Institute of Arthritis, Diabetes, and Digestive and Kidney DiseasesNational Institutes of HealthMarylandUSA

Personalised recommendations