Abstract
Receptors for insulin and insulinlike growth factors are present in target cells in extremely low quantities. They comprise only a small fraction of a percentage of the total membrane protein. Methods useful for their detection and characterization must therefore be exquisitely sensitive and specific. The high affinity and specificity of the hormones themselves, which are requisite for their biological function, provide the basis for one group of extensively used methods for investigating these receptors. Antibodies directed against these receptors provide another group of reagents with the necessary specificity and sensitivity. For certain purposes, antibodies have clear advantages over the hormones themselves. While hormone binding requires that the native conformation of the receptor be intact, many antibodies will react with the receptors even after they are denatured. Furthermore, receptors for insulin and insulinlike growth factors are large molecules with multiple domains. While insulin and the insulinlike growth factors react with their receptors at a single domain with a specificity that is fixed by nature, it is possible to select different antibodies that react with different sites on the receptors and exhibit different properties. It is therefore at least theoretically possible to use antibodies to map out different domains on the receptor and to learn something about their function. Monoclonal antibodies, because they recognize a single epitope, are ideal for this purpose. However, polyclonal antisera have also been useful. This chapter describes a series of antibodies that react with insulin and insulinlike growth factor-I receptors as well as information acquired by their use.
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Hier, J.S., Kahn, C.R., Roth, J., and Bar, R.S., 1975, Science 190: 63–65.
Flier, J.S., Kahn, C.R., Jarrett, D.B., and Roth, J., 1976, J. Clin. Invest. 58: 1442–2449.
Flier, J.S., Kahn, C.R., Jarret, D.B., and Roth, J., 1977, J. Clin. Invest. 60: 784–794.
Jarrett, D.B., Roth, J., Kahn, C.R., and Flier, J.S., 1976, Proc. Natl. Acad. Sci. USA 73: 4115–4119.
Kasuga, M., Hedo, J.A., Yamada, K.M., and Kahn, C.R., 1982, J. Biol. Chem. 257: 10392–10399.
Van Obberghen, E., Kasuga, M., LeCam, A., Hedo, J. A., Itin, A., and Harrison, L.C., 1981, Proc. Natl. Acad. Sci. USA 78: 1052–1056.
Hedo, J.A., Kasuga, M., Van Obberghen, E., Roth, J., and Kahn, C.R., 1981, Proc. Natl. Acad. Sci. USA 78: 4791–4795.
Maron, R., Kahn, C.R., Jacobs, S., and Fugita-Yamaguchi, Y., 1984, Diabetes 33: 923–928.
Jacobs, S., Chang, K.-J., and Cuatrecasas, P., 1978, Science 200: 1283–1284.
Jacobs, S., Hazum, E., and Cuatrecasas, P., 1980, J. Biol. Chem. 255: 6937–6940.
Kull, F.C., Jr., Jacobs, S., Su, Y.-F., and Cuatrecasas, P., 1982, Biochem. Biophys. Res. Commun. 106: 1019–1026.
Kull, F.C., Jr., Jacobs, S., Su, Y.-F., Svoboda, M.E., Van Wyk, J.J., and Cuatrecasas, P., 1983, J. Biol. Chem. 258: 6561–6566.
Bennett, A., Daly, F.T., and Hintz, R.L., 1981, Diabetes 30 (Suppl. 1): 55A.
Jacobs, S., Kull, F.C., Jr., and Cuatrecasas, P., 1983, Proc. Natl. Acad. Sci. USA 80: 1228–1231.
Roth, R.A., Cassell, D.J., Wong, K.Y., Maddux, B.A., and Goldfine, I.D., 1982, Proc. Natl. Acad. Sci. USA 79: 7312–7316.
Roth, R.A., Mesirow, M.L., and Cassell, D.J., 1983, J. Biol. Chem. 258: 14456–14460.
Fujita-Yamaguchi, Y., Choi, S., Sakamoto, Y., and Itakura, K., 1983, J. Biol. Chem. 258: 5045–5049.
Harrison, L.C., Flier, J.S., Roth, J., Karlsson, F.A., and Kahn, C.R., 1979, J. Clin. Endocrinol. Metab. 48: 59–65.
Kahn, C.R., Baird, K.L., Jarrett, D.B., and Flier, J.S., 1978, Proc. Natl. Acad. Sci. USA 75: 4209–4213.
Rosen, O.M., Chia, G.H., Fung, C., and Rubin, C.S., 1979, J. Cell Physiol. 99: 37–42.
Kasuga, M., Akanuma, Y., Tsushima, F., Iwamoto, Y., Kosoka, K., Kibata, M., and Kawanishi, K., 1978, Diabetes 27: 938–945.
Le Marchand-Brustel, Y., Gorden, P., Flier, J.S., Kahn, C.R., and Freychet, P., 1978, Diabetologia 14: 311–317.
Zierler, K., and Rogus, E.M., 1981, Clin. Res. 29: A579.
Van Obberghen, E., Spooner, P.M., Kahn, C.R., Chernick, S.S., Garrison, M.M., Karlsson, F.A., and Grunfeld, C., 1979, Nature 280: 500–502.
King, G.L., Kahn, C.R., Rechler, M.M., and Nissley, S.P., 1980, J. Clin. Invest. 66: 130–140.
Nagarajan, L., and Anderson, W.B., 1982, Biochem. Biophys. Res. Commun. 106: 974–980.
Posner, B.I., Bergeron, J.J.M., Josefsberg, Z., Khan, M.N., and Khan, R.J., 1981, Recent Prog. Horm. Res. 37: 539–82.
Carpentier, J.-L., Van Obberghen, E., Gorden, P., and Orci, L., 1981, Exp. Cell Res. 134: 81–92.
Goldfine, I.D., Jones, A.L., Hradek, G.T., and Wong, K.Y., 1981, Endocrinology 108: 1821–1828.
Vigneri, R., Goldfine, I.D., Wong, K.Y., Smith, G.J., and Pezzino, V., 1978, J. Biol. Chem. 253: 2098–2103.
Horvat, A., Li, E., and Katsoyannis, P.G., 1975, Biochim. Biophys. Acta 382: 609–620.
Goldfine, I.D., Vigneri, R., Cohen, D., Pliam, N.B., and Kahn, C.R., 1977, Nature 269: 698–700.
Patel, B.A., and Posner, B.I., 1983, Can. J. Biochem. Cell Biol. 61: 657–661.
Seals, J.R., and Jarett, L., 1980, Proc. Natl. Acad. Sci. USA 77: 77–81.
Petruzzelli, L.M., Ganguly, S., Smith, C.J., Cobb, M.H., Rubin, C.S., and Rosen, O.M., 1982, Proc. Natl. Acad. Sci. USA 79: 6792–6796.
Roth, R.A., Cassell, D.J., Maddux, B.A., and Goldfine, I.D., 1983, Biochem. Biophys. Res. Commun. 115: 245–252.
Roth, R.A., Maddux, B.A., Cassell, D.J., and Goldfine, I.D., 1983, J. Biol. Chem. 258: 12094–12097.
Kasuga, M., Carpentier, J.-L., Van Obberghen, E., Orci, L., and Gorden, P., 1983, Biochem. Biophys. Res. Commun. 114: 230–233.
Rosenfeld, R.G., Baldwin, D., Jr., Dollar, L.A., Hintz, R.L., Olefsky, J.M., and Rubenstein, A., 1981, Diabetes 30: 979–982.
Jonas, H.A., Baxter, R.C., and Harrison, L.C., 1982, Biochem. Biophys. Res. Commun. 109: 463–470.
Kasuga, M., Sasaki, N., Kahn, C.R., Nissley, S.P., and Rechler, M.M., 1983, J. Clin. Invest. 72: 1459–1469.
Armstrong, G.D., Hollenberg, M.D., Bhaumick, B., Bala, R.M., and Maturo, J.M. III, 1983, Can. J. Biochem. Cell Biol. 61: 650–656.
Roth, R.A., Maddux, B., Wong, K.Y., Styne, D.M., Vliet, G.V., Humbel, R.E., and Goldfine, I.D., 1983, Endocrinology 112: 1865–1867.
Yip, C.C., Moule, M.L., and Yeung, C.W.T., 1980, Biochem. Biophys. Res. Commun. 96: 1671–1678.
Heidenreich, K.A., Zahniser, N.R., Berhanu, P., Brandenburg, D., and Olefsky, J.M., 1983, J. Biol. Chem. 258: 8527–8530.
Podlecki, D.A., Frank, B.H., Kao, M., Horikoshi, H., Freidenberg, G., Marshall, S., Ciaraldi, T., and Olefsky, J.M., 1983, Diabetes 32: 697–704.
Jacobs, S., and Cuatrecasas, P., 1980, J. Clin. Invest. 66: 1424–1427.
Massague, J., and Czech, M.P., 1982, J. Biol. Chem. 257: 6729–6738.
Schweitzer, J.B., Smith, R.M., and Jarett, L., 1980, Proc. Natl. Acad. Sci. USA 77: 4692–4696.
McElduff, A., and Eastman, C.J., 1981, J. Recept. Res. 2: 87–95.
Zick, Y., Kasuga, M., Kahn, C.R., and Roth, J., 1983, J. Biol. Chem. 258: 75–80.
Zick, Y., Grunberger, G., Podskalny, J.M., Moncada, V., Taylor, S.I., Gorden, P., and Roth, J., 1983, Biochem. Biophys. Res. Commun. 116: 1129–1135.
Jacobs, S., Sahyoun, N.E., Saltiel, A.R., and Cuatrecasas, P., 1983, Proc. Natl. Acad. Sci. USA 80: 6211–6213.
Nishizuka, Y., and Takai, Y., 1981, Cold Spring Harbor Conf. Cell Prolif. 8: 237–249.
Castagna, M., Takai, Y., Kaibuchi, K., Sano, K., Kikkawa, U., and Nishizuka, Y., 1982, J. Biol. Chem. 257: 7847–7851.
Niedel, J.E., Kuhn, L.J., and Vandenbark, G.R., 1983, Proc. Natl. Acad. Sci. USA 80: 36–40.
Grunberger, G., and Gorden, P., 1982, Am. J. Physiol. 243: E319 — E324.
Thomopoulos, P., Testa, U., Gourdin, M.T., Hervey, C., Titeux, M., and Vainchenker, W., 1982, Eur. J. Biochem. 129: 389–393.
Rouis, M., Thomopoulos, P., Postel-Vinay, M.-C., Testa, U., Guyda, J.J., and Posner, B.I., 1984, Mol. PHysiol. 5:123–130.
Kasuga, M., Zick, Y., Blith, D.L., Karlsson, F.A., Haring, H.U., and Kahn, C.R., 1982, J. Biol. Chem. 257: 9891–9894.
Kasuga, M., Akanuma, Y., Tsushima, T., Suzuki, K., Kosaka, K., and Kibata, M., 1978, J. Clin. Endocrinol. Metab. 47: 66–77.
Lawrence, J.C. Jr., Lamer, J., Kahn, C.R., and Roth, J., 1978, Mol. Cell Biochem. 22: 153–157.
Baldwin, D., Jr., Terris, S., and Steiner, D.F., 1980, J. Biol. Chem. 255: 4028–4034.
Caro, J.F., and Amatruda, J.M., 1981, Am. J. Physiol. 240: E325 — E332.
Smith, C.J., Rubin, C.S., and Rosen, O.M., 1980, Proc. Natl. Acad. Sci. USA 77: 2641–2645.
Mukherjee, S.P., Attaway, E.J., and Mukherjee, C., 1982, Biochem. /nt. 4: 305–314.
Nicholas, K.R., and Topper, Y.J., 1983, Biochem. Biophys. Res. Commun. 111: 988–993.
Caro, J.F., and Amatruda, J.M., 1980, Science 210: 1029–1031.
Koontz, J.W., 1984, Mol. Cell. Biochem. 58: 139–146.
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Jacobs, S. (1985). Immunochemical Characterization of Receptors for Insulin and Insulinlike Growth Factor-I. In: Czech, M.P. (eds) Molecular Basis of Insulin Action. Springer, Boston, MA. https://doi.org/10.1007/978-1-4684-4874-0_2
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DOI: https://doi.org/10.1007/978-1-4684-4874-0_2
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