Fatty Acid Effects on Sarcoplasmic Reticulum Function in Vitro
When heart muscle is rendered ischemic or hypoxic, changes in lipid and phospholipid metabolism result in the accumulation of amphiphilic compounds, including non-esterified fatty acids (FA), fatty acylcarnitine esters, and lysophosphatides (1–5). These amphiphilic substances, possibly by interacting with membrane lipids, can alter the functional properties of a variety of membrane systems in vitro; and this interaction has been postulated as a possible mechanism for ischemic heart muscle dysfunction in vivo. (See references 6–8 for reviews). The mechanism by which amphiphiles alter membrane function in vitro, however, remains unclear. The calcium sequestration properties and ATPase activity of sarcoplasmic reticulum membrane vesicles (SR) isolated from skeletal muscle are modified by both non-esterified fatty acids and acylcarnitines (9–11). The present report describes studies examining the effect of oleic and palmitic acids on the properties of calcium sequestration and ATPase activity of SR in order to elucidate the mechanisms by which structurally dissimilar fatty acids can alter differently SR calcium sequestration.
KeywordsOleic Acid ATPase Activity Palmitic Acid Sarcoplasmic Reticulum Sarcoplasmic Reticulum Membrane
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