Monoclonal Antibodies as Cell Targeted Carriers of Covalently and Non-Covalently Attached Toxins
The strategy of using cell specific monoclonal antibodies to modify the indiscriminate binding characteristics of lethal toxins has stimulated considerable interest because of its therapeutic potential. Intact plant or bacterial toxins linked to antibodies may acquire some cell selectivity but because toxin binding sites are retained, non-specific toxicity can still occur. Since the binding region of such toxins is distinct from the enzymatically active toxic portion, these components can be cleaved and isolated (Olsnes and Pihl, 1973). Thus, purified toxic subunits can be coupled to antibodies to produce highly selective cytotoxic agents which are devoid of residual toxin binding.
KeywordsDaudi Cell Chain Conjugate Bovine Gamma Globulin Human Gamma Globulin Rabbit Reticulocyte Lysate System
Unable to display preview. Download preview PDF.
- Jansen, F.K., Blythman, H.E., Carriere, D., Casellas, P., Gros, O., Gros, P., Laurent, J.C., Paolucci, F., Pau, B., Poncelet, P., Richer, G., Vidal, H. and Voisin, G.A., 1982, Immunotoxins: hybrid molecules combining high specificity and potent cytotoxicity, Immunol. Rev., 62:185.PubMedCrossRefGoogle Scholar
- Klinnman, N.R., Pickard, A.R., Sigal, N.H., Gearhart, P.J., Metcalf, E.S., and Pierce, S.K., 1976, Assessing B cell diversification by antigen receptor and precursor cell analysis, Ann. Immunol., 127C:489.Google Scholar
- Olsnes, S., Sandvig, K., Refsnes, K. and Pihl, A., 1976, Rates of different steps involved in the inhibition of protein synthesis by the toxic lectins abrin and ricin, J. Biol. Chem., 257:3985.Google Scholar