Alteration of Liposomal-Surface Properties with Synthetic Glycolipids
Liposomes have been proposed as carriers to introduce biologically active agents into cells, and their various applications have been extensively investigated.1–3 The tissue distribution and clearance kinetics of drug-containing liposomes are known to be affected by phospholipid composition, size and surface charge.4,5 Small liposomes, were shown to be better than larger liposomes for specific delivery of their contents to target cells in-vivo.6 Although successes have been amply demonstrated for in-vitro targeting of liposomes including covalent attachment of proteins onto the surface of liposomes,7,8 no in-vivo specificity has yet been reported for any liposomes thus modified. The interaction of liposomes with cells in-vivo occurs in quite a complex biological milieu and the binding of proteins from this milieu may also have important effects on liposome behaviour.9 Our approach to improve liposomes as a drug-delivery system is to use small synthetic glycolipids as cell-surface ligands to alter the tissue distribution of the modified liposomes in various tissues and organs.
KeywordsMouse Peritoneal Macrophage Carbohydrate Determinant Plasma High Density Lipoprotein Liposomal Surface Control Liposome
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