Toxic Conjugates of Epidermal Growth Factor and Asialofetuin
Our laboratory has, for a number of years, been interested in the role and mode of action of polypeptide growth factors. These agents bind to specific cell surface receptors and initiate a cascade of biological responses leading either to cell division or expression of a new phenotype as a consequence of ligand-receptor interactions. In many cases one of the most notable initial responses is ligand-induced endocytosis of the receptor-ligand complex, with eventual degradation of the ligand and, in some cases, degradation of the receptor as well. As one of several approaches (Herschman et al., 1982) to the study of polypeptide hormone/growth factor induced responses, we have constructed heteroconjugates of binding ligands with protein toxins or their enzymic portions.
KeywordsEpidermal Growth Factor Receptor Epidermal Growth Factor A431 Cell Diphtheria Toxin Hepatocyte Culture
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