Heterogeneity of Extramitochondrial Forms of Aspartate Aminotransferase and Malate Dehydrogenase in Yoshida Ascites Hepatoma Cells

  • Pier Paolo Gazzaniga


Isozyme patterns of experimental both solid and ascites rat hepatomas have been extensively investigated in recent years. For many enzymes, such as hexokinase (Sato and Sugimura, 1973), pyruvate kinase (Farina et al., 1974), alcohol dehydrogenase (Cederbaum and Rubin, 1976), glycogen phosphorilase (Sato et al., 1976), acid and alkaline phosphatases (Emmelot and Bos,1969; Kaneko et al., 1972), malate dehydrogenase (Otani and Morris, 1971), aspartate and alanine aminotransferase (Otani and Morris, 1971), tyrosine aminotransferase (Aviram and Hershko, 1977), S-adenosylmethionine synthetase (Liau et al., 1979; Tsukada and Okada, 1980) evidence has been given that the disappearance of some enzymatic components that are typical of the normal adult rat liver and appearance of new, frequently foetal-type isozymes generally correspond to the degree of dedifferentiation and to the growth rate of the tumor.


Acid Phosphatase Pyruvate Kinase Malate Dehydrogenase Isozyme Pattern Atypical Form 


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    Aviram M. and Hershko A., 1977: Interconversion of multiple forms of tyrosine aminotransferase in vitro and in vivo in cultured hepatoma cells. Bioch. Bioph. Acta, 498, 83.CrossRefGoogle Scholar
  2. 2.
    Cederbaum A.J. and Rubin E., 1976: Kinetic properties of alcohol dehydrogenase in hepatocellular carcinoma and normal tissues of rat. Cancer Res., 36, 2274.PubMedGoogle Scholar
  3. 3.
    Emmelot P. and Bos C.J., 1969: A survey of enzyme activities displayed by plasma membranes isolated from normal and preneoplastic livers and primary and transplanted hepatomas of the rat. Int. J. Cancer, 4, 705.PubMedCrossRefGoogle Scholar
  4. 4.
    Farina F.A., Shatton J.B., Morris H.P. and Weinhouse S., 1974: Isozymes of pyruvate kinase in liver and hepatomas of the rat. Cancer Res., 34, 1439.PubMedGoogle Scholar
  5. 5.
    Gazzaniga P.P., 1972: Rat liver isozymes in cloudy swelling. Enzyme, 14, 25.Google Scholar
  6. 6.
    Gazzaniga P.P., 1975: Rat liver isozymes in acute carbon tetrachloride and ethionine poisoning. Enzyme, 20, 193.PubMedGoogle Scholar
  7. 7.
    Gazzaniga P.P., 1979: Rat liver isozymes in acute and chronic thioacetamide poisoning. Arch. De Vecchi, 63, 1.Google Scholar
  8. 8.
    Kakizoe T., Kawachi T. and Sugimura T., 1976: ß -glucuronidase isozyme patterns of experimental hepatomas of rats. Gann, 671 289.Google Scholar
  9. 9.
    Kaneko A., Dempo K., Onoè T. and Isaka H., 1972: Deviations in isozyme patterns of acid phosphatase and esterase and in ultra-structures of Yoshida ascites hepatomas from rat hepatocytes. Gann, 63, 747.PubMedGoogle Scholar
  10. 10.
    Liau M.C., Chang C.F. and Becker F.F., 1979: Alteration of Sadenosylmethionine synthetases during chemical hepatocarcinogenesis and in resulting carcinomas. Cancer Res., 39, 2113.PubMedGoogle Scholar
  11. 11.
    Otani T.T. and Morris H.P., 1971: Comparison of activity and isozyme patterns of four enzymes from hepatomas of different growth rates. J. Nat. Cancer Inst., 47, 1247.PubMedGoogle Scholar
  12. 12.
    Sato K., Satoh K., Sato T., Imai F. and Morris H.P., 1976: Isozyme patterns of glycogen phosphorilase in rat tissues and transplantable hepatomas. Cancer Res., 36, 487.PubMedGoogle Scholar
  13. 13.
    Sato S. and Sugimura T., 1973: Presence of fast-moving type IV hexokinase isozyme in Morris hepatoma. Gann, 64, 359.PubMedGoogle Scholar
  14. 14.
    Tsukada K. and Okada G., 1980: S-adenosylmethionine synthetase isozyme patterns from rat hepatoma induced by N-2-fluorenylacetamide. Bioch. Bioph. Res. Commun., 94, 1078.CrossRefGoogle Scholar

Copyright information

© Plenum Press, New York 1984

Authors and Affiliations

  • Pier Paolo Gazzaniga
    • 1
  1. 1.Institute of General PathologyUniversity La SapienzaRomeItaly

Personalised recommendations