Cell-Type-Independent Accumulation of Phosphatidic Acid Induced by Trifluoperazine in Stimulated Human Platelets, Leukocytes, and Fibroblasts
It is generally believed that receptor-stimulated breakdown of phosphatidylinositol (PI) is implicated in transmembrane cell signaling whereby activation of a variety of cell surface receptors brings about a rise in intracellular calcium (Michell, 1975; Nishizuka, 1983; Irvine et al., 1983). The breakdown of polyphosphated phosphoinositides is characterized by the rapid interconversion of short-lived intermediates (suggesting the so-called “phosphatidylinositol cycle”), where diacylglycerol (DG) is phosphorylated to phosphatidic acid (PA) by a DG-kinase, which is in turn reconverted to PI via CDP-diglyceride-inositolphosphatidyl transferase.
KeywordsPhosphatidic Acid Phorbol Ester Phosphatidic Acid Human Foreskin Fibroblast Phenyl Methyl Sulfonyl Fluoride
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