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Molecular Biology of HTLV

  • F. Wong-Staal
  • G. Franchini
  • B. Hahn
  • S. Arya
  • E. P. Gelmann
  • V. Manzari
  • R. C. Gallo

Abstract

HTLV is a family of related, human T-cell tropic retroviruses, consisting of at least two distinct subgroups, HTLV-I and HTLV-II. The biology, seroepidemiology and protein biochemistry of HTLV are presented by R. C. Gallo et al. elsewhere in this symposium. This paper will present only molecular biological studies on HTLV, focusing on two aspects:
  1. (1)

    A molecular epidemiological survey of human leukemias using cloned HTLV probes. Seroepidemiological studies have already defined geographical areas where HTLV is endemic or present sporadically, as well as shown an association of HTLV with a subtype of mature T-cell malignancy now collectively called adult T-cell leukemia-lymphoma (ATLL). When molecular clones of the two HTLV subgroups became available, we conducted a survey of DNA from different leukemic cells, both fresh and cultured, for the presence of HTLV sequences. This analysis will allow detection of HTLV infection even in the absence of viral antigens or antibodies. In seropositive cases where the patient’s diseases are not ATLL, this analysis may indicate whether HTLV plays a direct role in these diseases.

     
  2. (2)

    The molecular mechanism of transformation by HTLV in vivo and in vitro. We approach this by examining the state of the provirus in fresh ATLL cells, primary T-cell lines established from these, and normal cord blood T-cell lines transformed in vitro with HTLV and the mode of expression of viral and some relevant cellular genes in different HTLV-infected cells.

     

Keywords

Hairy Cell Leukemia Fresh Peripheral Blood Defective Provirus Fresh Leukemic Cell Molecular Epidemiological Survey 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

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Copyright information

© Plenum Press, New York 1984

Authors and Affiliations

  • F. Wong-Staal
    • 1
  • G. Franchini
    • 1
  • B. Hahn
    • 1
  • S. Arya
    • 1
  • E. P. Gelmann
    • 1
  • V. Manzari
    • 2
  • R. C. Gallo
    • 1
  1. 1.Laboratory of Tumor Cell Biology, National Cancer InstituteNational Institutes of HealthBethesdaUSA
  2. 2.Istituto di Patologia Generale III CattedraUniversità degli Studi di RomaItaly

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