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Rearrangement and Abnormal Expression of Human c-myc in Acute Lymphocytic Leukemia

  • C. Peschle
  • F. Mavilio
  • N. M. Sposi
  • A. Giampaolo
  • A. Caré
  • L. Bottero
  • M. Bruno
  • G. Mastroberardino
  • R. Gastaldi
  • M. G. Testa
  • G. Alimena
  • S. Amadori
  • F. Mandelli

Abstract

We have studied a case of acute lymphocytic leukemia (L3 type) with 8;14 chromosome translocation. DNA obtained from peripheral blood leukemic cells was analyzed by restriction endonuclease mapping and hybridization with genomic or c-DNA human c-myc probes. The breakpoint of the translocation has been localized within the first intron of the c-myc gene,thus leaving the first untranslated exon on chromosome 8q− and rearranging the whole protein-coding region in the IgH locus on chromosome 14q+. Northern blot analysis showed high levels of two different c-myc transcripts originated from the translocated gene, which were not detected in WBC in remission phase. These studies demonstrate for the first time rearrangement and abnormal expression of a cellular one-gene (c-myc) in primary cells from an acute leukemia patient.

Keywords

Leukemic Cell Acute Lymphocytic Leukemia Remission Phase Acute Leukemia Patient Untranslated Exon 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. 1).
    Neel, B.G., Jhanwar, S.C., Chagauti, R.S.K. and Hayward, W.S., (1982) Proc. Natl. Acad. Sci. USA 79, 7842–7846.PubMedCrossRefGoogle Scholar
  2. 2).
    Dalla Favera, R., Bregni, M., Erikson, J., Patterson, D., Gallo, R.C. and Croce, C.M. (1982) Proc. Natl. Acad. Sci. USA 79, 7824–7827.CrossRefGoogle Scholar
  3. 3).
    Westin, E.H., Wong-Staal, F., Gelmann, E.P., Dalla Favera, R., Papas, T.S., Lauterberger, J.A., Eva, A., Reddy, E.P., Tronick, S.R., Aaronson, S.A. and Gallo, R.C. (1982) Proc. Natl. Acad. Sci. USA 79, 2490–2494PubMedCrossRefGoogle Scholar
  4. 4).
    Dalla Favera, R., Wong-Staal, F. and Gallo, R.C. (1982) Nature, 299, 61–63.CrossRefGoogle Scholar
  5. 5).
    Taub, R., Kirsch, I., Morton, C., Lenoir, G., Swan, D., Tronick S., Aaronson, S. and Leder, P. (1982) Proc. Natl. Acad. Sci. USA 79, 7837–7841.PubMedCrossRefGoogle Scholar
  6. 6).
    Dalla Favera, R., Martinotti, S., Gallo, R.C., Erikson, J. and Croce C.M. (1983) Science 219, 963–967.CrossRefGoogle Scholar
  7. 7).
    Adams, J.M., Gerondakis, S., Webb, E., Corcoran, L.M., and Cory, S. (1983) Proc. Natl. Acad. Sci. USA 80, 1982–1986.PubMedCrossRefGoogle Scholar
  8. 8).
    Hamlyn, P.H. and Rabbitts, T.H. (1983) Nature 304, 135–139.PubMedCrossRefGoogle Scholar
  9. 9).
    Battey, J., Moulding, C., Taub, R., Murphy, W., Stewart, T.,Potter, H., Lenoir, G. and Leder, P. (1983) Cell 34, 779–787.PubMedCrossRefGoogle Scholar
  10. 10).
    Croce, C.M., Thierfelder, W., Erikson, J., Nishikura, K., Finan, J., Lenoir, G.M. and Nowell, P.C. (1983). Proc. Natl. Acad. Sci. USA 80, 6922–6926.PubMedCrossRefGoogle Scholar
  11. Erikson, J., ar-Rushdi, A., Drwinga, H.L., Nowell, P.C. and Croce, C.M. (1983) Proc. Natl. Acad. Sci. USA 80, 820–824. 820–824.Google Scholar
  12. 12).
    Nishikura, K., ar-Rushdi, A., Erikson, J., Watt, R., Rovera, G., and Croce, C.M. (1983) Proc. Natl. Acad. Sci USA 80, 4822–4826.PubMedCrossRefGoogle Scholar
  13. 13).
    ar-Rushdi, A., Nishikura, K., Erikson, J., Watt, R., Rovera, G. and Croce, C.M. (1983) Science 222, 390–393.PubMedCrossRefGoogle Scholar
  14. 14).
    Perry, (1983) Cell, 33, 647–649.PubMedCrossRefGoogle Scholar
  15. 15).
    Leder, P., Battey, J., Lenoir, G., Moulding, C., Murphy, W., Potter, H., Stewart, T. and Taub, R. (1983) Science 222, 765–771.PubMedCrossRefGoogle Scholar
  16. 16).
    Mavilio, F., Giampaolo, A., Caré, A., Sposi, N.M. and Marinucci, M. (1983) Blood, 62, 230–233.PubMedGoogle Scholar
  17. 17).
    Mavilio, F., Giampaolo, A., Caré, A., Migliaccio, G., Calandrini, M., Russo, G., Pagliardi, G.L., Mastroberardino, G., Marinucci, M. and Peschle, C. (1983) Proc. Natl. Acad. Sci. USA 80, 6907–6911.PubMedCrossRefGoogle Scholar
  18. 18).
    Watt, R., Nishikura, K., Sorrentino, J., ar-Rushdi, A., Croce,C.M. and Rovera, G. (1983) Proc. Natl. Acad. Sci. USA 80, 6307–6311.PubMedCrossRefGoogle Scholar
  19. 19).
    Maniatis, T., Fritsch, E.F. and Sambrook, J. Molecular Cloning A Laboratory Manual. Cold Spring Harbor. (1982) New York.Google Scholar
  20. 20).
    Robertson, M. (1983) Nature, 306, 733–736.PubMedCrossRefGoogle Scholar

Copyright information

© Plenum Press, New York 1984

Authors and Affiliations

  • C. Peschle
    • 1
  • F. Mavilio
    • 1
  • N. M. Sposi
    • 1
  • A. Giampaolo
    • 1
  • A. Caré
    • 1
  • L. Bottero
    • 1
  • M. Bruno
    • 1
  • G. Mastroberardino
    • 2
  • R. Gastaldi
    • 2
  • M. G. Testa
    • 2
  • G. Alimena
    • 3
  • S. Amadori
    • 3
  • F. Mandelli
    • 3
  1. 1.Dept. of HematologyIstituto Superiore di SanitàItaly
  2. 2.Istituto Patologia Medica (VI)Italy
  3. 3.Chair of HematologyUniversity of RomeItaly

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