Nucleotide Sequences Homologous to a Cloned Repeated Human DNA Fragment in Human Leukemic DNA’s

Abstract

The human genome contains repetitive nucleotide sequences that are in part organized in blocks in the heterochromatin and partly interspersed among single copy genes. It is known that chromosome deletions and rearrangements occur frequently in leukemias and cancers at cytogenetic analysis. Methods of genome investigation such as analysis of DNA by restriction enzyme digestion and Southern blot hybridization could demonstrate finer genome rearrangements. Human total DNA centrifuged to equilibrium in Ag⁺-Cs₂SO₄ shows four satellite DNAs and a shoulder on the light side. From this shoulder a DNA fraction, by us previously called homogeneous main band DNA¹, made up mainly by highly repeated DNA² other than satellite DNAs, can be separated by a subsequent CsCl centrifugation.