Nucleotide Sequences Homologous to a Cloned Repeated Human DNA Fragment in Human Leukemic DNA’s

  • Luca Ceccherini Nelli
  • Gianmarco Corneo


The human genome contains repetitive nucleotide sequences that are in part organized in blocks in the heterochromatin and partly interspersed among single copy genes. It is known that chromosome deletions and rearrangements occur frequently in leukemias and cancers at cytogenetic analysis. Methods of genome investigation such as analysis of DNA by restriction enzyme digestion and Southern blot hybridization could demonstrate finer genome rearrangements. Human total DNA centrifuged to equilibrium in Ag+-Cs2SO4 shows four satellite DNAs and a shoulder on the light side. From this shoulder a DNA fraction, by us previously called homogeneous main band DNA1, made up mainly by highly repeated DNA2 other than satellite DNAs, can be separated by a subsequent CsCl centrifugation.


Acute Myeloid Leukemia Chronic Myeloid Leukemia Satellite DNAs Restriction Enzyme Digestion Acute Lymphatic Leukemia 
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  1. 1.
    G. Corneo, E. Ginelli and E. Polli, Renaturation properties and localization in heterochromatin of human satellite DNAs, Biochim. Biophys. Acta 247: 528 (1971).PubMedGoogle Scholar
  2. 2.
    G. Corneo, D. Meazza, M. Bregni, P. Tripputi and L. Ceccherini Nelli, Restriction enzyme studies on human highly repeated DNAs, Experientia 38: 454 (1982).PubMedCrossRefGoogle Scholar
  3. 3.
    A. Maresca and M. F. Singer, Deca-satellite: a highly polymorphic satellite that joins oCsatellite in the African green monkey genome, J. Mol. Biol. 164: 493 (1983).PubMedCrossRefGoogle Scholar
  4. 4.
    R. Kominami, Y. Urano, Y. Mishima, M. Muramatsu, K. Moriwaki and H. Yoshikura, Novel repetitive sequence families showing size and frequency polymorphism in the genomes of mice, J. Mol. Biol. 165: 209 (1983).PubMedCrossRefGoogle Scholar

Copyright information

© Plenum Press, New York 1984

Authors and Affiliations

  • Luca Ceccherini Nelli
    • 1
  • Gianmarco Corneo
    • 1
  1. 1.Cattedra di Patologia Medica IIUniversità di di MilanoMilanoItaly

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