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Immunological Detection of Cellular Targets for V-onc Gene Coded Tyrosine Kinases

  • F. G. Giancotti
  • M. F. Di Renzo
  • P. C. Marchisio
  • G. Tarone
  • G. Tarone
  • L. Naldini
  • S. Giordano
  • P. M. Comoglio

Abstract

The induction of transformed phenotype by retrovirus-infected cells is triggered by the action of unique genetic sequences, named onc genes. The protein products coded by a family of viral onc genes — v-src, v-ros, v-fps, v-yes, v-abl, v-fes — are known to be phosphokinases endowed with the unusual property of phosphorylating tyrosine residues (1). Since phosphotyrosine (P-TYR) constitutes less than 0.1% of the phosphoaminoacids in normal cells and it is only 5 to 10 fold increased in virally transformed cells, identification of cellular substrates for tyrosine kinases has been hampered by difficulties in distinguishing proteins containing P-TYR from those containing only phosphoserine (P-SER) and phosphothreonine (P-THR)(2).

Keywords

Rous Sarcoma Virus Immunological Detection Adhesion Plaque Avian Sarcoma Virus Detergent Insoluble Fraction 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Plenum Press, New York 1984

Authors and Affiliations

  • F. G. Giancotti
    • 1
  • M. F. Di Renzo
    • 1
  • P. C. Marchisio
    • 1
  • G. Tarone
    • 1
  • G. Tarone
    • 1
  • L. Naldini
    • 1
  • S. Giordano
    • 1
  • P. M. Comoglio
    • 1
  1. 1.Department of Histology, School of MedicineUniversity of TorinoTorinoItaly

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