Advertisement

Modulating Effects of Thymic Factors on Natural Cell-Mediated Reactivities of Natural and Cyclophosphamide-Treated Mice

  • Francesco Bistoni
  • Manuela Baccarini
  • Lucia Scaringi
  • Rosanna Mazzolla
  • Paolo Puccetti
  • Pierfrancesco Marconi

Abstract

Our laboratory has been concerned for some time with the problem of the effects of in vivo administration of thymic extracts to mice on the expression of natural cell-mediated cytotoxicity against both fungal (1) and neoplastic cells (2) in intact as well as immunodepressed hosts. This seems particularly important because of the recent interest in the possiblc therapeutic use of thymus extracts (3, 4) and in consideration of the possible role of natural immunity in resistance mechanisms against neoplasia and microbial infections (5, 6). The issue is even more relevant when dealing with compromised hosts that are known to experience an increased susceptibility to infections and development of malignancies. In the present paper we report on the effects of thymus extract administrations in intact or cyclophosphamide treated mice on in vitro cell-mediated cytotoxicity against radiolabelled YAC-1 cells (7) and C. albicans particles (8).

Keywords

Candida Albicans Thymus Extract Charles River Breeding Laboratory Thymic Factor Candidacidal Activity 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    F. Bistoni, M. Baccarini, E. Blasi, C. Riccardi, P. Marconi, E. Garaci, Modulation of polymorphonucleate-mediated cytotoxicity against Candida albicans by thymosino α1, Thymus, in press.Google Scholar
  2. 2.
    F. Bistoni, M. Baccarini, P. Puccetti, P. Marconi, E. Garaci, Enhancement of natural killer cell activity in mice by treatment with a thymic factor, Cancer Immunol. Immunother., submitted for publication.Google Scholar
  3. 3.
    M. H. Cohen, P. B. Chretien, D. C. Ihde, B. E. Fossieck, R. Mazuch, P. A. Bunn, A. V. Johnston, S. E. Shackney, M. J. Matthews, S. D. Lipson, D. E. Kenady, J. D. Minna, Thymosin fraction V and intensive combination chemotherapy prolonging the survival of patients with small-cell lung cancer, J. Am. Med. Assoc. 241: 1813 (1979).CrossRefGoogle Scholar
  4. 4.
    J. Shoham, E. Theodor, H.J. Brenner, B. Goldman, A. Lusky, S. Chaitchick, Enhancement of the immune system of chemotherapy-treated cancer patients by simultaneous treatment with thymic extract TP-1, Cancer Immunol. Immunother. 9: 173 (1980).CrossRefGoogle Scholar
  5. 5.
    G. Cudckowicz, M. Landy, G. M. Shearer, Natural resistance systems against foreign cells, tumours and microbes, Academic Press, N. Y. (1978).Google Scholar
  6. 6.
    R. B. Herberman, H. T. Holden, Natural cell-mediated immunity, in: “Advances in Cancer Research”, G. Klein, S. Weinhouse, eds., Academic Press, N. Y., vol. 27, p. 305 (1978).Google Scholar
  7. 7.
    R. K. Oldham, J. R. Ortaldo, H. T. Holden, R. B. Herberman, Direct comparison of three isotopic release microassay as measures of cell-mediated immunity to virus-induced lymphoma in rats, J. Natl. Cancer Inst. 58: 1061 (1977).PubMedGoogle Scholar
  8. 8.
    F. Bistoni, M. Baccarini, E. Blasi, P. Puccetti, P. Marconi, A radiolabel release microassay for phagocytic killing of Candida albicans, J. Immunol.Methods 52: 369 (1982).PubMedCrossRefGoogle Scholar
  9. 9.
    R. B. Herberman, M. E. Nunn, D. H. Lavrin, Natural cytotoxic reactivity of mouse lymphoid cells. I. Distribution of reactivity and specificity, Int. J. Cancer 16: 216 (1975).Google Scholar
  10. 10.
    M. Baccarini, E. Blasi, P. Puccetti, F. Bistoni, Phagocytic killing of Candida albicans by different murine effectors, Sabouraudia in press.Google Scholar
  11. 11.
    A. S. Klein, J. Shoham, Effect of the thymic factor thymostimulin (TP-1) on the survival of tumor-bearing mice, Cancer Res. 41: 3217 (1981).PubMedGoogle Scholar
  12. 12.
    C. Y. Lau, G. Goldstein, Functional effect of thymopoietin32–36 (TP-5) on cytotoxic lymphocytes precursors units (CLP-U). I. Enhancement of splenic CLP-U in vitro and in vivo after suboptimal antigenic stimulation, J. Immunol. 124: 1861 (1980).PubMedGoogle Scholar
  13. 13.
    P. Puccetti, A. Santoni, C. Riccardi, H. T. Holden, R. B. Herber-man, Activation of mouse macrophages by pyran copolymer and role in augmentation of natural. killer activity, Int. J. Cancer 24: 819 (1979).PubMedCrossRefGoogle Scholar

Copyright information

© Plenum Press, New York 1984

Authors and Affiliations

  • Francesco Bistoni
    • 1
  • Manuela Baccarini
    • 1
  • Lucia Scaringi
    • 1
  • Rosanna Mazzolla
    • 1
  • Paolo Puccetti
    • 2
  • Pierfrancesco Marconi
    • 1
  1. 1.Institute of Medical MicrobiologyUniversity of PerugiaPerugiaItaly
  2. 2.Institute of PharmacologyUniversity of PerugiaPerugiaItaly

Personalised recommendations