The Information Content of Single Channel Data
Single channel signals were initially observed in artificial bilayer membranes (Bean et al., 1969; Gordon and Haydon, 1972; Hladkey and Haydon, 1972). They were first seen in biological membranes using the patch clamp technique (Neher and Sakmann, 1976). A number of excellent reviews have detailed this technique, including gigohm (>109 ohm) seals and ultra-high resolution recordings, as well as the many different types of preparations and channels from which recordings have been made (Neher et al., 1978; Hamill et al., 1981). In this chapter I will examine a different aspect of this subject. I seek to clarify the reasons for recording single channels, instead of measuring the mean behavior of a population of channels (whole cell recording), or the fluctuations caused by the random activity of such a population.
KeywordsSingle Channel Closed Time Single Channel Conductance Single Channel Current Channel Open Time
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- Labarca, P. P., and Miller, C., 1981, A K+ -selective, three state channel from fragmented sarcoplasmic reticulum of frog leg muscle, J. Memb. Biol., 61:31.Google Scholar
- Patlak, J. B., 1983, Conditional probability measurements on two models of Na cbannel kinetics, in: “The Physiology of Excitable Cells,” A. Grinnell and W. Moody, editors. Liss, Inc., New York.Google Scholar