The major approaches used for detection of major histocompatibility complex (MHC)-encoded antigens involve serologic methods; there are serologically detected determinants that are recognized on essentially all cells of the body and others that have an apparently more restricted tissue distribution.(1–3) In addition, there are cellular methods, studying activation of T lymphocytes in mixed leukocyte culture (MLC), that can be used to define MHC-encoded determinants. Here, also, determinants can be divided into two categories including those that activate much of the proliferative response in a primary mixed-leukocyte culture in which the donors of the responding and stimulating cells differ by an entire MHC, and those that are recognized by cytotoxic T lymphocytes.(4)
KeywordsMajor Histocompatibility Complex Serologic Method Normal Human Bone Marrow Mixed Leukocyte Culture Cellular Method
Unable to display preview. Download preview PDF.
- 1.Terasaki PI (ed): Histocompatibility Testing 1970. Copenhagen, Munksgaard, 1970.Google Scholar
- 2.Kissmeyer-Nielsen F (ed): Histocompatibility Testing 1975. Copenhagen, Munksgaard, 1975.Google Scholar
- 3.Bodmer WF, Batchelor Jr, L, Bodmer JG, Festenstein H, Morris PJ (eds): Histocompatibility Testing 1977. Copenhagen, Munksgaard, 1978.Google Scholar
- 5.Mempel W, Grosse-Wilde H, Baumann P, Netzel B, Steinbauer-Rosenthal I: Population genetics of the MLC response: Typing for MLC determinants using homozygous and heterozygous reference cells. Transpl Proc 5:1529, 1973.Google Scholar
- 9.Reinsmoen NL, Noreen HJ, Sasazuki T, Segali M, Bach FH: Roles of HLA-DR and HLA-D antigens in haplotype-primed LD typing reagents, in Kaplan JG (ed): The Molecular Basis of Immune Cell Function, Proceedings of the 13th International Leucocyte Culture Conference. Amsterdam, Elsevier/North-Holland, 1979.Google Scholar