Application of the Microbiological Approach to the Study of Passive Monovalent Salt Transport in a Kidney Epithelial Cell Line, MDCK

  • Milton H. SaierJr.


The mammalian kidney is a complex organ consisting of several tissues and a large number of distinct cell types. A multiplicity of epithelial cell types line fluid-filled tubules, differing morphologically and functionally depending on the segment of the tubule in which they arise. They are connected by junctional complexes which make for tissue integrity and allow intercellular communication (Fig. 1). They are polar, possessing plasma membranes of differing protein compositions on the mucosal and serosal sides of the cell. Asymmetry is essential to epithelial cell function since different transport systems are associated with the two membranes. Underlying the epithelial cell layer is a basement membrane, a complex network of proteins and carbohydrate-rich macromolecules, which confer a relatively static shape to the tubule. Underlying the basement membrane are fibroblasts which together with the epithelial cells participate in basement membrane biogenesis, synthesize collagen, and comprise much of the tissue bulk. Muscle, nerve, and endothelial cells participate in organ mobility, communication, and nutrition, respectively. The complexity of the kidney is awe inspiring. An understanding of its function will require the concerted efforts of numerous investigators applying a variety of experimental approaches to the same problem.


MDCK Cell Athymic Nude Mouse Kidney Epithelial Cell Salt Transport Mock Cell 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. Boerner, P., and Saier, M. H., Jr., 1982a, Nutrient transport and growth regulation in kidney epithelial cells (MDCK) cultured in a defined medium, Cold Spring Harbor Conferences on Cell Proliferation 9:555–565.Google Scholar
  2. Boerner, P., and Saier, M. H., Jr., 1982b, Growth regulation and amino acid transport in epithelial cells: I. Influence of culture conditions and transformation on A, ASC, and L transport activities, J. Cell Physiol. 12:240–246.CrossRefGoogle Scholar
  3. Chuman, L., Fine, L. G., Cohen, A. H., and Saier, M. H., Jr., 1982, Continuous growth of proximal tubular kidney epithelial cells in hormone-supplemented serum-free medium, J. Cell Biol. 94:506–510.PubMedCrossRefGoogle Scholar
  4. Erlinger, S., and Saier, M. H., Jr., 1982, Decrease in protein content and cell volume of cultured dog kidney epithelial cells during growth. Importance for transport measurements, In Vitro 18:196–202.PubMedCrossRefGoogle Scholar
  5. McRoberts, J. A., Taub, M., and Saier, M. H., Jr., 1981, The Madin—Darby canine kidney (MDCK) cell line, in: Functionally Differentiated Cell Lines (G. Sato, ed.), Alan R. Liss, New York, pp. 117–139.Google Scholar
  6. McRoberts, J. A., Erlinger, S., Rindler, M. J., and Saier, M. H., Jr., 1982, Furosemide-sensitive salt transport in the Madin-Darby canine kidney cell line: Evidence for the cotransport of Na+, K+, and CI, J. Biol. Chem. 257: 2260–2266.PubMedGoogle Scholar
  7. McRoberts, J. A., Tran, C. T., and Saier, M. H., Jr., 1983, Characterization of low potassium-resistant mutants of the Madin—Darby canine kidney cell line with defects in NaCl/KCl symport, J. Biol. Chem. 258:12320–12326.PubMedGoogle Scholar
  8. Newman, M. J., Foster, D. L., Wilson, T. H., and Kaback, H. R., 1981, Purification and reconstitution of functional lactose carrier from Escherichia coli, J. Biol. Chem. 256:11804–11808.PubMedGoogle Scholar
  9. Racker, E., Violand, B., O’Neal, S., Alfonzo, M., and Telford, J., 1979, Reconstitution, a way of biochemical research; some new approaches to membrane-bound enzymes, Arch. Biochem. Biophys. 198:470–477.PubMedCrossRefGoogle Scholar
  10. Rindler, M. J., and Saier, M. H., Jr., 1981, Evidence for Na+/H+ antiport in cultured dog kidney cells (MDCK), J. Biol. Chem. 256:10820–10825.PubMedGoogle Scholar
  11. Rindler, M. J., Chuman, L. M., Shaeffer, L., and Saier, M. H., Jr., 1979a, Retention of differentiated properties in an established dog kidney epithelial cell line (MDCK), J. Cell Biol. 81:635–648.PubMedCrossRefGoogle Scholar
  12. Rindler, M. J., Taub, M., and Saier, M. H., Jr., 1979b, Uptake of 22Na+ by cultured dog kidney cells (MDCK), J. Biol. Chem. 254: 254:11431–11439.PubMedGoogle Scholar
  13. Rindler, M. J., McRoberts, J. A., and Saier, M. H., Jr., 1982, (Na+,K+ )-cotransport in the Madin—Darby canine kidney cell line: Kinetic characterization of the interaction between Na+ and K+, J. Biol. Chem. 257:2254–2259.PubMedGoogle Scholar
  14. Saier, M. H., Jr., 1979, Sugar transport mediated by the bacterial phosphoenolpyruvate-dependent phosphotransferase system, in: Microbiology, American Society for Microbiology, Washington, D.C., pp. 72–75.Google Scholar
  15. Saier, M. H., Jr., 1981, Growth and differentiated properties of a kidney epithelial cell line (MDCK), Am. J. Physiol. 240:C106–C109.PubMedGoogle Scholar
  16. Saier, M. H., Jr., and Boyden, D., 1984, Mechanism, regulation and physiological significance of the loop diuretic-sensitive NaCl/KCl symport system in animal cells, Molecular and Cellular Biochemistry 59:11–32.PubMedCrossRefGoogle Scholar
  17. Saier, M. H., Jr. Erlinger, S., and Boerner, P., 1982, Studies on growth regulation and the mechanism of transformation of the kidney epithelial cell line, MDCK: Importance of transport function to growth, in: Membranes in Growth and Development (J. F. Hoffman, ed.), in press.Google Scholar
  18. Stiles, C. D., Desmond, W., Chuman, L. M., Sato, G., and Saier, M. H., Jr., 1976a, Growth control of heterologous tissue culture cells in the congenitally athymic nude mouse, Cancer Research 36:1353–1360.PubMedGoogle Scholar
  19. Stiles, C. D., Desmond, W., Chuman, L. M., Sato, G., and Saier, M. H., Jr., 1976b, Relationship of cell growth behavior in vitro to tumorigenicity in athymic nude mice, Cancer Research 36:3300–3305.PubMedGoogle Scholar
  20. Stiles, C. D., Roberts, P. E., Saier, M. H., Jr., and Sato, G., 1977, Growth regulation and suppression of metastasis in the congenitally athymic nude mouse, in: Modern Trends in Human Leukemia II (R. Neth, R. C. Gallo, K. Mannweiler, and W. C. Maloney, eds.), Lehmanns Verlag, Munchen, pp. 185–194.Google Scholar
  21. Taub, M., 1978, Isolation of amiloride-resistant clones from dog kidney epithelial cells, Somatic Cell Genetics 4:609–616.PubMedCrossRefGoogle Scholar
  22. Taub, M., and Saier, M. H., Jr., 1978, An established but differentiated kidney epithelial cell line (MDCK), in: Methods in Enzymology, Volume LVIII, Academic Press, New York, pp. 552–560.Google Scholar
  23. Taub, M., and Saier, M. H., Jr., 1979, Regulation of 22Na+ transport by calcium in an established kidney epithelial cell line, J. Biol. Chem. 254:11440–11444.PubMedGoogle Scholar
  24. Taub, M., and Saier, M. H., Jr., 1981, Amiloride-resistant Madin—Darby canine kidney (MDCK) cells exhibit decreased cation transport, J. Cell Physiol. 106:191–199.PubMedCrossRefGoogle Scholar
  25. Taub, M., Chuman, L., Saier, M. H., Jr., and Sato, G., 1979, Growth of Madin—Darby canine kidney epithelial cell (MDCK) line in hormone supplemented, serum-free medium, Proc. Natl. Acad. Sci. USA 76:3338–3342.PubMedCrossRefGoogle Scholar
  26. Taub, M., U., B., Chuman, L., Rindler, M. J., Saier, M. H., Jr., and G. Sato, 1981, Alterations in growth requirements of kidney epithelial cells in defined medium associated with malignant transformation, J. Supra. Struc. Cellular Biochem. 15:63–72.CrossRefGoogle Scholar
  27. U, H. S., Saier, M. H., Jr., and Ellisman, M. H., 1979, Tight junction formation is closely linked to the polar redistribution of intramembranous particles in aggregating MDCK epithelia, Exp. Cell Res. 122:384–390.PubMedCrossRefGoogle Scholar
  28. U, H. S., Saier, M. H., Jr., and Ellisman, M. H., 1980, Tight junction formation in the establishment of intramembranous particle polarity in aggregating MDCK cells. Effect of drug treatment, Exper. Cell Res. 128:223–235.CrossRefGoogle Scholar

Copyright information

© Plenum Press, New York 1985

Authors and Affiliations

  • Milton H. SaierJr.
    • 1
  1. 1.Department of BiologyUniversity of CaliforniaSan Diego, La JollaUSA

Personalised recommendations