Electrical Properties of MDCK Cells
The electrical characterization of a transporting epithelium requires information three main aspects: (1) the overall electrical properties across the whole cell layer; (2) the transcellular permeation route; and (3) the paracellular pathway. In the present article we describe assumptions, techniques, efforts, and information collected on these aspects of the monolayer of MDCK cells, which under certain culturing conditions behave as a natural transporting epithelium (Misfeldt et al., 1976; Cereijido et al., 1978a,b; Valentich et al., 1979). Furthermore, we also describe our efforts to relate the electrical properties to different structural features (e.g., the strands of the occluding junctions) and to intracellular organelles (e.g., the cytoskeleton), both under steady-state conditions and under transient conditions such as the establishment of the junctions, the removal of Ca2+, and the addition of test substances such as cytochalasin and colchicine.
KeywordsMDCK Cell Paracellular Pathway Kind Permission Mock Cell Transcellular Route
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