Importance of Hormonally Defined, Serum-Free Medium for in Vitro Studies Concerning Epithelial Transport
Hormonally defined, serum-free media have been developed for a number of different types of epithelial cell lines. Some of these cell lines, such as the MDCK and the LLC—PK1 pig kidney cell line, have been demonstrated to possess the capacity for transepithelial solute transport in vitro. MDCK and LLC—PK1 cells are of particular interest to study in this regard, as their primary function in vivo is to reabsorb solutes. However, the transport properties of many other epithelial cell lines are also of inferest. Although many of the epithelial cells in such cultures possess differentiated functions that are not primarily reabsorptive in nature, these cells often must transport particular solutes across the cell layer, in order to carry out their primary differentiated functions. For example, thyroid and testicular epithelial cells possess the capacity for trans-epithelial transport of select solutes in vivo. Such transepithelial transport ultimately permits the secretion of thyroid hormone in the thyroid and facilitates germ cell maturation in the testes.
KeywordsEpithelial Cell Line Madin Darby Canine Kidney Madin Darby Canine Kidney Cell Kidney Epithelial Cell Epithelial Cell Culture
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