Inducers of Dome Formation in Epithelial Cell Cultures including Agents That Cause Differentiation
Epithelia are cell sheets that line body cavities such as the lumina of the intestine, kidney tubules, and salivary glands. The differentiated phenotype of epithelia involves structural and functional specializations of the plasma membrane which enable these cells to transport ions and fluids across the cell layer. The plasma membrane of this cell type is polarized into distinct apical and basolateral regions which differ functionally, morphologically, and biochemically. Occluding junctions form between adjacent cells at the apical/basolateral boundary and can regulate the passage of ions across the cell layer. In addition, occluding junctions have been proposed to maintain cell polarity by serving as a barrier to lateral diffusion within the membrane of intrinsic proteins from basolateral and apical domains. The Na+/K+ ATPase of epithelia is restricted to the basolateral surface. Na+ pump polarity is believed to create the driving force for vectorial transport of Na+ across the cell layer, setting up an osmotic gradient which drives an accompanying net water flux in the basolateral direction.
KeywordsMammary Epithelial Cell MDCK Cell Erythroid Differentiation Kidney Epithelial Cell Epithelial Cell Culture
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