Vitamin D Activates (Na+-K+)ATPase: A Possible Regulation of Phosphate and Calcium Uptake by Cultured Embryonic Chick Small Intestine

  • Heide S. Cross
  • Meinrad Peterlik
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 178)


The effect of vitamin D3 and of its biologically most active metabolite, 1,25-dihydroxyvifamin D3, on intestinal calcium and inorganic phosphate (Pi) absorptive mechanisms has been studied extensively in an organ culture system of embryonic chick small intestine (Corradino, 1973; Peterlik, 1978; Cross and Peterlik, 1982). Elevation of Ca and Pi uptake by vitamin D depends on prior gene activation by the sterol (cf. also Franceschi and DeLuca, 1981b). However, this vitamin D action is only partially expressed in undifferentiated absorptive cells, since they respond to the sterol solely by an increase in Ca but not in Pi uptake. Induction of the latter has been shown to require advanced target cell maturation (Cross and Peterlik, 1982).


Embryonic Chick Sodium Pump Jejunal Segment Intestinal Brush Border Membrane Vesicle Contralateral Plasma Membrane 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. Berner, W., Kinne, R., and Murer, H.: Phosphate Transport into Brush-Border Membrane Vesicles Isolated from Rat Small Intestine. Biochem.J. 160: 467–474 (1976).PubMedGoogle Scholar
  2. Birge, S.J., and Alpers, D.H.: Stimulation of Intestinal Mucosal Proliferation by Vitamin D. Gastroenterol. 64: 977–982 (1973).Google Scholar
  3. Charney, A.N., Gots, R.E., and Gianella, R.A.: (Na+-K+)-Stimulated Adenosine Triphosphatase in Isolated Intestinal Villus Tip and Crypt Cells. Biochim.Biophys.Acta 367: 265–270 (1974).PubMedCrossRefGoogle Scholar
  4. Corradino, R.A.: Embryonic chick intestine in organ culture: A unique system for the study of the intestinal calcium absorptive mechanism. J.Cell Biol. 58: 64–78 (1973).PubMedCrossRefGoogle Scholar
  5. Corradino, R.A.: A Simple Technique for the Measurement of Unidirectional Calcium Influx at the Mucosal Surface of Organ-Cultured Embryonic Chick Duodenum. Analyt.Biochem. 91: 60–69 (1978).PubMedCrossRefGoogle Scholar
  6. Cross, H.S., and Peterlik, M.: Differential Response of Enterocytes to Vitamin D during Embryonic Development: Induction of Intestinal Inorganic Phosphate, D-Glucose and Calcium Uptake. Horm.metabol.Res. 14: 649–652 (1982).CrossRefGoogle Scholar
  7. Franceschi, R.T., and De Luca, H.F.: Characterization of 1,25-Dihydroxyvitamin D3-dependent Calcium Uptake in Cultured Embryonic Chick Duodenum. J.Biol.Chem. 256: 3840–3847 (1981a).PubMedGoogle Scholar
  8. Franceschi, R.T., and De Luca, H.F.: The effect of inhibitors of protein and RNA synthesis on la,25-dihydroxyvitamin D3-dependent calcium uptake in cultured embryonic chick duodenum. J.Biol.Chem. 256: 3848–3852 (1981b).PubMedGoogle Scholar
  9. Fuchs, R., and Peterlik, M.: Vitamin D-Induced Phosphate Transport in Intestinal Brush Border Membrane Vesicles. Biochem. Biophys.Res.Comm. 93: 87–92 (1980).PubMedCrossRefGoogle Scholar
  10. Hildmann, B., Schmidt, A., and Murer, H.: Ca++-Transport across Basal-Lateral Plasma Membranes from Rat Small Intestinal Epithelial Cells. J.Membrane Biol. 65: 55–62 (1982).CrossRefGoogle Scholar
  11. Peterlik, M.: Phosphate transport by embryonic chick duodenum: Stimulation by vitamin D3. Biochim.Biophys.Acta 514: 164–171 (1978).PubMedCrossRefGoogle Scholar
  12. Peterlik, M., Fuchs, R., and Cross, H.S.: Stimulation of D-glucose transport. A novel effect of vitamin D on intestinal membrane transport. Biochim.Biophys.Acta 649: 138–142 (1981).PubMedCrossRefGoogle Scholar
  13. Rozengurt, E., and Mendoza, S.: Monovalent Ion Fluxes and the Control of Cell Proliferation in Cultured Fibroblasts. Ann.N.Y. Acad.Sci. 339: 175–190 (1980).CrossRefGoogle Scholar
  14. Schultz, S.G.: Transport Across Small Intestine, in: “Membrane Transport in Biology” Vol. IVB, Giebisch, G., Tosteson, D.C., Ussing, H.H., eds., Springer Verlag New York (1979).Google Scholar
  15. Wasserman, R.H.: Intestinal absorption of calcium and phosphorus. Fed.Proc. 40: 68–72 (1981).PubMedGoogle Scholar

Copyright information

© Plenum Press, New York 1984

Authors and Affiliations

  • Heide S. Cross
    • 1
  • Meinrad Peterlik
    • 1
  1. 1.Department of General and Experimental PathologyUniversity of ViennaAustria

Personalised recommendations